GENERAL INFORMATION ........................... 1. Dataset title: Dataset interaction NNV-sea bream, NNV-sea bass 2. Authors:M. Carmen Alonso, Esther Garcia-Rosado, Julia Bejas, Juan Gemez-Mata, Patricia Moreno 3. Author contact information: M. Carmen Alonso (mdalonso@uma.es) METHODOLOGICAL INFORMATION ................................. 1. Description of the methods for collection/generation of data: - Itramuscular injection of NNV isolates (high and low virulence) in European sea bass and Gilthead sea bream. Brain and kidney were sampled at several times post-infection and processed for RNA extraction and gene transcription analyses 2. Data processing methods - Sampling - RNA extraction, cDNA sysnthesis and PCR amplification - mRNA transcription of immunogenes related with IFN-I system, apoptosis and inflammation -Viral genome quantification by absolute qPCR 3. Software or instruments needed to interpret the data: - Statistical analysis: data analyses were performed with GraphPad software GraphPad -PCR amplifications were onducted with Lightcycler 96 thermocycler (Roche Diagnostics, S.L.U, Swiss) 4. Standards and calibration information, if appropriate: Not applicable 5. Environmental or experimental conditions: - Virus propagation and cell culture: E-11 cells were grown in Leibovitz L-15 medium (Gibco, Paisley, UK) supplemented with 10% foetal bovine serum (FBS, Gibco, Paisley, UK), 100 unit/mL penicillin and 10 mg/mL streptomycin (Sigma) at 25 °C until confluence. The viral isolates SpDl-IAusc965.09 (DlNNV, RGNNV genotype) and SaNNV (RGNNV/SJNNV reassortant) were propagated in E-11 cell in L-15 medium supplemented with 2% FBS, 100 units/mL penicillin and 10 mg/mL streptomycin at 25 ºC -Fish maintenance: fish were maintained under the following conditions for 30 days: 24 ± 0.5 ºC, pH 7.5 ± 0.5, salinity 38 ± 1 ppt, oxygen 6 ± 1 mg/l, NO2 < 3 mg/l, light 12 h. FILE OVERVIEW ---------------------- The dataset is distributed in 13 files in .xlsx format. This provides information on: - Sea bass cortisone assay, tnf-alpha transcription (.xlsx) - Sea bass inflammation assay, IL-1beta transcription (.xlsx) - Sea bass inflammation assay, IL-8 transcription (.xlsx) - Sea bass inflammation assay, tnf-alpha transcription (.xlsx) - Sea bream in vivo hsp70 (.xlsx) - Sea bream in vivo IL-1beta (.xlsx) - Sea bream in vivo IL-6 (.xlsx) - Sea bream in vivo IL-10 (.xlsx) - Sea bream in vivo RTP3 (.xlsx) - Sea bream in vivo irf3 (.xlsx) - Sea bream in vivo sacsin (.xlsx) - Sea bream in vivo tnf-alpha (.xlsx) - Viral genome quantification RNA1 (.xlsx) DATA-SPECIFIC INFORMATION: ------------------------------------------- 1. Name File: Sea bass cortisone assay, tnf-alpha transcription 1.1. Variables list: Variable name: injection Description: fish were injected with LPS, cortisone (10 and 20 uM) or PBS (control) Units of measure or value labels: Quantitative values ​​expressed as Cq (tnf-alpha and endogenous gen, 18S) Variable name: sampling time Units of measure or value labels: days after injection 1.2. Codes or symbols: No specific codes have been used 1.3. Special formats or abbreviations used: Not applicable 2. Name File: Sea bass inflammation assay, IL-1beta transcription (.xlsx) 2.1. Variables list: Variable name: injection Description: fish were injected with L-15, RGNNV (DlNNV), Mut247+270RGNNV (Mut247+270DlNNV) Units of measure or value labels: Quantitative values ​​expressed as Cq (IL-1beta and endogenous gen, 18S) Variable name: sampling time Units of measure or value labels: days after injection 2.2. Codes or symbols: Code or symbol: Mut247+270RGNNV is an RGNNV isolate with aminoacids SJNNV-type in positions 247 and 270 of the CP 2.3. Special formats or abbreviations used: Not applicable 3. Name File: Sea bass inflammation assay, IL-8 transcription (.xlsx) 3.1. Variables list: Variable name: injection Description: fish were injected with L-15, RGNNV (DlNNV), Mut247+270RGNNV (Mut247+270DlNNV) Units of measure or value labels: Quantitative values ​​expressed as Cq (IL-8 and endogenous gen, 18S) Variable name: sampling time Units of measure or value labels: days after injection 3.2. Codes or symbols: Code or symbol: Mut247+270RGNNV is an RGNNV isolate with aminoacids SJNNV-type in positions 247 and 270 of the CP 3.3. Special formats or abbreviations used: Not applicable 4. Name File: Sea bass inflammation assay, tnf-alpha transcription (.xlsx) 4.1. Variables list: Variable name: injection Description: fish were injected with L-15, RGNNV (DlNNV), Mut247+270RGNNV (Mut247+270DlNNV) Units of measure or value labels: Quantitative values ​​expressed as Cq (tnf-alpha and endogenous gen, 18S) tnf-alpha Variable name: sampling time Units of measure or value labels: days after injection 4.2. Codes or symbols:Mut247+270RGNNV is an RGNNV isolate with aminoacids SJNNV-type in positions 247 and 270 of the CP 4.3. Special formats or abbreviations used: Not applicable 5. Name File: Sea bream in vivo hsp70 5.1. Variables list: Variable name: injection Description: fish were injected with L-15, RGNNV (DlNNV), RGNNV/SJNNV (SaNNV) Units of measure or value labels: quantitative values ​​expressed as Cq (hsp70 and endogenous gen, beta-actin) Variable name: sampling time Units of measure or value labels: days after injection 5.2. Codes or symbols: No specific codes have been used 5.3. Special formats or abbreviations used: Not aplicable 6. Name File: Sea bream in vivo IL-1beta 6.1. Variables list: Variable name: injection Description: fish were injected with L-15, RGNNV (DlNNV), RGNNV/SJNNV (SaNNV) Units of measure or value labels: quantitative values ​​expressed as Cq (IL-1beta and endogenous gen, beta-actin) Variable name: sampling time Units of measure or value labels: days after injection 6.2. Codes or symbols: No specific codes have been used 6.3. Special formats or abbreviations used: Not aplicable 7. Name File: Sea bream in vivo IL-6 7.1. Variables list: Variable name: injection Description: fish were injected with L-15, RGNNV (DlNNV), RGNNV/SJNNV (SaNNV) Units of measure or value labels: quantitative values ​​expressed as Cq (IL-6 and endogenous gen, beta-actin) Variable name: sampling time Units of measure or value labels: days after injection 7.2. Codes or symbols: No specific codes have been used 7.3. Special formats or abbreviations used: Not aplicable 8. Name File: Sea bream in vivo IL-10 8.1. Variables list: Variable name: injection Description: fish were injected with L-15, RGNNV (DlNNV), RGNNV/SJNNV (SaNNV) Units of measure or value labels: quantitative values ​​expressed as Cq (IL-10 and endogenous gen, beta-actin) Variable name: sampling time Units of measure or value labels: days after injection 8.2. Codes or symbols: No specific codes have been used 8.3. Special formats or abbreviations used: Not aplicable 9. Name File: Sea bream in vivo irf3 9.1. Variables list: Variable name: injection Description: fish were injected with L-15, RGNNV (DlNNV), RGNNV/SJNNV (SaNNV) Units of measure or value labels: quantitative values ​​expressed as Cq (irf3 and endogenous gen, beta-actin) Variable name: sampling time Units of measure or value labels: days after injection 9.2. Codes or symbols: No specific codes have been used 9.3. Special formats or abbreviations used: Not aplicable 10. Name File: Sea bream in vivo rtp3 10.1. Variables list: Variable name: injection Description: fish were injected with L-15, RGNNV (DlNNV), RGNNV/SJNNV (SaNNV) Units of measure or value labels: quantitative values ​​expressed as Cq (rtp3 and endogenous gen, beta-actin) Variable name: sampling time Units of measure or value labels: days after injection 10.2. Codes or symbols: No specific codes have been used 10.3. Special formats or abbreviations used: Not aplicable 11. Name File: Sea bream in vivo sacsin 11.1. Variables list: Variable name: injection Description: fish were injected with L-15, RGNNV (DlNNV), RGNNV/SJNNV (SaNNV) Units of measure or value labels: quantitative values ​​expressed as Cq (sacsin and endogenous gen, beta-actin) Variable name: sampling time Units of measure or value labels: days after injection 11.2. Codes or symbols: No specific codes have been used 11.3. Special formats or abbreviations used: Not aplicable 12. Name File: Sea bream in vivo tnf-alpha 12.1. Variables list: Variable name: injection Description: fish were injected with L-15, RGNNV (DlNNV), RGNNV/SJNNV (SaNNV) Units of measure or value labels: quantitative values ​​expressed as Cq (tnf-alpha and endogenous gen, beta-actin) Variable name: sampling time Units of measure or value labels: days after injection 12.2. Codes or symbols: No specific codes have been used 12.3. Special formats or abbreviations used: Not aplicable 13. Name File: Viral genome quantification RNA1 13.1. Variables list: Variable name: injection Description: fish were injected with RGNNV (DlNNV), RGNNV/SJNNV (SaNNV) Units of measure or value labels: quantitative values ​​expressed as Cq (RNA1) Variable name: sampling time Units of measure or value labels: days after injection 13.2. Codes or symbols: No specific codes have been used 13.3. Special formats or abbreviations used: Not aplicable ... MORE INFORMATION These data derived from the project "Interplay between nodavirus virulence and innate response in sea bass and sea bream (INTERNODASS)" (Proyectos de I+D+i Retos Investigación, PID2020-115954RB-I00), and it has been published in Gemez-Mata et al. (2024) Comparative analysis of immune gene transcription in sea bream (Sparus aurata) challenged with RGNNV or RGNNV/SJNNV betanodaviruses. Pathogens, 13(6), 478 (https://doi.org/10.3390/pathogens13060478) ------------------- [Include any other information about the dataset that is not reflected in this template and that you consider relevant. Incluya cualquier otra información sobre el conjunto de datos que no haya quedado reflejada en esta plantilla y que considere relevante]