GENERAL INFORMATION 
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1. Dataset title: Feeding trial in Chelon labrossum with Rugulopteryx okamurae

2. Authors:
Salvador Arijo Andrade (University of Málaga) sarijo@uma.es
Roberto Abdala Díaz (University of Málaga)



METHODOLOGICAL INFORMATION 
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1. Description of the methods for collection/generation of data: 

A feeding trial was carried out on the Chelon labrossum fed with feed supplemented at 5% and 15% with Rugulopteryx okamurae algae extract. After 30 days of feeding, the expression of different genes in liver, intestine and head kidney was evaluated.The data were processed using the PLAFF method and obtained the fold change value.



DATA-SPECIFIC INFORMATION:
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FILE NAME: Dataset-Feed_challenge.xls

1. Sheet name: "RNA_samples"

Variable		Description

Processing date	        Processing date
Sample code		Sample code
Sample Type		RNA
Tank number		Tank number
Fish number		Fish number
Organ 			Gut, liver or head kidney
[RNA] 		        Obtained RNA concentration 
260/280		        RNA quality			
260/230                 RNA quality


2. Sheet name: "Liver_qPCR_data"

Variable		Description

Sample name		Sample liver name
cDNA code		cDNA code
Cq values		Cq values in every gen analized 
Average			Average Ratio PFAFFL obtained for every treatment        
Desvest			Standart deviation Ratio PFAFFL obtained for every treatment			



3. Sheet name: "Head_kidney_qPCR_data"

Variable		Description

Sample name		Sample head kidney name
cDNA code		cDNA code
Cq values		Cq values in every gen analized 
Average			Average Ratio PFAFFL obtained for every treatment        
Desvest			Standart deviation Ratio PFAFFL obtained for every treatment			

4. Sheet name: "Gut_qPCR_data"

Variable		Description

Sample name		Sample Gut name
cDNA code		cDNA code
Cq values		Cq values in every gen analized 
Average			Average Ratio PFAFFL obtained for every treatment        
Desvest			Standart deviation Ratio PFAFFL obtained for every treatment			

