Differences in the viral induction of the senegalese sole Mx protein in RTG-2 and CHSE-214 cells

Abstract

Transfected RTG-2 cells infected with VHSV showed significant induction of the luciferase reporter gene, compared to the control non-infected cells, at 24, 48 and 72 h post infection (p.i.). The maximum expression was recorded at 72 h p.i. (2.25 folds compared to the control cells). In these cells, the infection with IPNV and EHNV did not result in the luciferase expression at any time tested. In transfected CHSE-214 cells, EHNV stimulated luciferase expression at 24 h p.i. (2.17 folds compared to the control cells), whereas cells infected with IPNV and VHSV did not show luciferase activity at any time. The lack of induction of the SsMx promoter after VHSV infection in CHSE-214 cells, as well as after EHNV infection in RTG-2 cells may be caused by viral IFN-suppression mechanisms, as has been demonstrated for IPNV in previous studies. Furthermore, the different induction of the SsMx promoter observed in RTG-2 and CHSE-214 cells after infection with the same virus indicates that cellular specific factors are involved in the IFN-signaling response. Therefore, the use of two different cellular systems might be an interesting approach to identify such cellular factors.