Vectors and P64k gene targeting for tandem affinity purification in Neisseria meningitidis

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dc.centroFacultad de Medicina
dc.contributor.authorBorroto Escuela, Dasiel Óscar
dc.contributor.authorAlea, Mileydis Perez
dc.contributor.authorFernandez, Wilber Romero
dc.contributor.authorGil, Daniel Bello
dc.date.accessioned2026-01-29T08:56:28Z
dc.date.issued2006-04-01
dc.departamentoFisiología Humana, Histología Humana, Anatomía Patológica y Educación Físico Deportiva
dc.description.abstractWe present and describe the construction of tagging cassettes and plasmids for tandem affinity purification (TAP) of proteins in Neisseria meningitidis. The tagging cassette is designed for carboxyl-terminal tagging of proteins and it contains only two repeats of IgG-binding units. P64k protein from N. meningitidis was chosen to fuse at these new affinity tags. This protein is well recognized in immunoassays by serum from human convalescent meningococcal disease and it is highly immunogenic in animals. To continue the characterization of this meningococcal antigen, we designed and constructed two vectors for use in TAP purification method. We also carried-out preliminary test to check the correct expression of the protein fused in these vectors.
dc.identifier.citationDasiel Oscar Borroto Escuela, Mileydis Perez Alea, Wilber Romero Fernandez, Daniel Bello Gil, Vectors and P64k gene targeting for tandem affinity purification in Neisseria meningitidis, Journal of Microbiological Methods, Volume 65, Issue 1, 2006, Pages 187-193, ISSN 0167-7012, https://doi.org/10.1016/j.mimet.2005.07.013. (https://www.sciencedirect.com/science/article/pii/S0167701205001958)
dc.identifier.doi10.1016/j.mimet.2005.07.013
dc.identifier.issn01677012
dc.identifier.urihttps://hdl.handle.net/10630/45003
dc.language.isoeng
dc.publisherElsevier
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectBacterias
dc.subjectMeningitis
dc.subject.otherNeisseria meningitidis
dc.subject.otherP64k
dc.subject.otherTandem affinity purification
dc.subject.otherVectors
dc.titleVectors and P64k gene targeting for tandem affinity purification in Neisseria meningitidis
dc.typejournal article
dc.type.hasVersionAM
dspace.entity.typePublication

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