Anticonvulsant-induced Toxic Epidermal Necrolysis: Monitoring the Immunologic Response.

Abstract

Background Toxic epidermal necrolysis is a severe reaction with skin involvement induced by different drugs and other agents. The mechanisms implicated in the induction of the reaction are poorly understood. Objective To study the involvement of T lymphocytes and other immunocompetent cells in the peripheral blood, blister fluid, and affected skin of 3 patients who developed a severe reaction after receiving anticonvulsant medication. Methods Quantification of T lymphocytes expressing the skin homing receptor (cutaneous lymphocyte-associated antigen [CLA]) in peripheral blood, skin and skin blister fluid and assessment of other adhesion molecules, activation markers and inflammatory interleukins by flow cytometry, immunohistochemistry and RT-PCR. Results An increase in CD3+CLA+ cells paralleling the severity of the disease was observed in both peripheral blood and skin, tending to normalize as soon as patient’s condition improved. E-selectin was detected in endothelial vessels in parallel with CLA expression on lymphocytes. An overexpression of TNF-, IFN- and IL-2 was also observed in peripheral blood mononuclear cells. The expression of the different markers changed over the course of the disease. Conclusions. These data show an increase in activated T cells expressing the skin-homing receptor in both tissue and peripheral blood accompanying clinical symptoms, with a recruitment of macrophages and an overexpression of cytokines. All these results suggest an important role for T cells in the production of toxic epidermal necrolysis.