Transcript profiling of wood transcriptional regulators, wood chemistry and phenotyping in poplar and eucalyptus genotypes growgin under drought, salinity and limiting nitrogen conditions

Abstract

The possibilities for using lignocellulosic biomass produced from forest crops in order to obtain second generation biofuels is a key issue in the current context of climate change and the promotion of renewable energy sources.

It is therefore important to make progress in the functional characterization of candidate genes (CGs) associated with wood traits relevant to bioenergy as well as to determine the effects of environmental factors (drought, salinity, and nitrogen availability among others) on wood production and chemistry. Fast growing species adapted to coppicing such as Populus and Eucalyptus spp. are among the most promising forest species for bioenergy production in the short term.

The aim of our project is to compare how three common environmental factors such as drought, salt and nitrogen availability, affect wood composition and transcript profiling of CGs involved in wood formation in different poplar and eucalypt genotypes. Poplar and eucalypt genotypes were selected based on their potential for bioenergy production and their performances under different abiotic stresses. In particular, four poplar genotypes: P. x canadensis (Oudenberg); P. tremula x P. alba; two P. alba clones (J 1-3-18 and Po10-10-20), and four eucalyptus genotypes: E. camaldulensis (169); E. urograndis (5E); E. globulus (Odiel); E. globulus (Anselmo) were used.

We found that the environmental factors caused responses of different magnitude and at different levels among the genotypes.

Correlation analysis will allow us to specifically associate changes in the expression of regulatory genes caused by environmental factors with alterations in secondary xylem composition and phenotypic adjustments.

Funding support: MINECO (SP)-PIM2010PKB-0702; TreeforJoules (Plant KBBE ANR(FR)-10-KBBE-0007; BMBF (G)-0315914A; FCT(PT) AGR-GPL/0001/2010. Some of the candidate genes were selected in the frame of the project ANR-06-GPLA-0007, 2006-2009. RTA2011-00006-00-00.