Biofilm inhibition of pathogenic strains by extracellular products (ECPS) of Shewanella.sp Probiotic

Abstract

Shewanella putrefaciens Pdp11 and V.proteolyticus DCF 12.2 are strains isolated by our research group. Recent works have been focused in the identification of substances as alternative of anti-biofilm methods and their implication in surface attachment inhibition. In this research, S. putrefaciens Pdp11 and V.proteolyticus DCF 12.2 have been cultured under different growth conditions (temperature, culture media and during 24 and 48 hours of incubation) and their extracellular products (ECPs) have been extracted and tested as potential postbiotics that affect the biofilm formation of several fish pathogenic strains. This assay results evidence that Pdp11_Pmix_2324 ECPs have showed the most impact in the biofilm formation of pathogenic strains. Therefore, ECPs secreted by Pdp11 and V.proteolyticus DCF 12.2 are implicated in the inhibition to adhesion of pathogens on surfaces.