Bone organoid generation based on double scaffolding.

Abstract

Decellularized bone matrix ( is a classic approach in bone tissue engineering based on the removal of bone tissue cells and the calcified phase using chemical, physical o enzymatic agents The resulting matrix preserves its three dimensional structure and biomechanical properties, providing a native microenvironment suitable for osteogenic development However, due to the complexity of the bone structure, with this method has not been possible to obtain an experimentally reproducible bone organoid that sufficiently replicates the bone biology Another approach to generate organoids is the use of hydrogels functionalized with adhesion peptides and morphogenetic proteins that favor the differentiation and osteogenic capacity of the organoid In this work we developed a new bone model based on the combination of hydrogels and DBMs We tested PVA and dextran hydrogels in combination with BMP responsive reporter cell line ( and the long term effect of the RGD peptide addition to hydrogels evaluating osteogenic differentiation by alkaline phosphatase method and luciferase assays We analyze by histology decellularized DBMs combined with dextran hydrogels containing BRITER cells cultured under osteogenic conditions for 3 weeks Our results showed that this double scaffolding is able to support osteogenic differentiation as well as osseointegration, proving its potential for bone organoid generation