RT Journal Article
T1 Molecular effect of fenofibrate on PBMC gene transcription related to lipid metabolism in patients with metabolic syndrome
A1 Moreno-Indias, Isabel
A1 Tinahones-Madueño, Francisco José
A1 Clemente-Postigo, María Mercedes
A1 Castellano-Castillo, Daniel
A1 Fernández-García, José Carlos
A1 Macías-González, Manuel
A1 Queipo-Ortuño, María Isabel
A1 Cardona-Díaz, Fernando
K1 Síndrome metabólico
K1 Lípidos - Metabolismo
AB Background: Both fasting and postprandial hypertriglyceridaemia are considered independent risk factors for atherosclerosis. Treatment of hypertriglyceridaemia is based on fibrates, which activate the peroxisome proliferator-activated receptor alpha (PPARα). However, the metabolic pathways that activate or inhibit fibrates, and how the postprandial triglyceride levels are modified, have not yet been fully described. Accordingly, the aim of this study was to determine the feasibility of peripheral blood mononuclear cells (PBMC) to study the effects of fenofibrate in patients with the metabolic syndrome.Materials and methods: A fat overload was given to 50 patients before and after treatment with fenofibrate for 3 months. Anthropometric and biochemical variables as well as gene expression in PBMC were analysed.Results: After treatment with fenofibrate, we observed a decrease in both baseline and postprandial (3 h after the fat overload) levels of serum triglycerides, cholesterol and uric acid and an increase in HDL cholesterol and apolipoprotein AI levels. After treatment, there was also a rise in PPARα and RXRα expression and changes in genes regulated by PPARα, both baseline and postprandial. Furthermore, in vitro experiments showed that a PPARα agonist changed the expression of genes related with lipid metabolism.Conclusion: Treatment with fenofibrate reduced fasting and postprandial serum triglyceride levels, possibly through a mechanism related with an increase in the expression of RXRα and PPARα, by activating the pathways involved in the uptake and degradation of triglycerides and increasing the synthesis of apolipoprotein. These results suggest that PBMC may be useful for the easy study of fenofibrate actions.
PB Wiley
YR 2017
FD 2017-06
LK https://hdl.handle.net/10630/45645
UL https://hdl.handle.net/10630/45645
LA eng
NO Moreno-Indias I, Tinahones FJ, Clemente-Postigo M, Castellano-Castillo D, Fernández-García JC, Macias-Gonzalez M, Queipo-Ortuño MI, Cardona F. Molecular effect of fenofibrate on PBMC gene transcription related to lipid metabolism in patients with metabolic syndrome. Clin Endocrinol (Oxf). 2017 Jun;86(6):784-790. doi: 10.1111/cen.13320. Epub 2017 Mar 27. PMID: 28251701.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 3 mar 2026