RT Journal Article
T1 Identification, resistance to antibiotics and biofilm formation of bacterial strains isolated from a reverse osmosis system of a drinking water treatment plant
A1 Borrego García, Juan José
A1 Labella Vera, Alejandro Manuel
A1 Molero, Rosana
A1 Leiva Rebollo, Rocío
A1 Pérez-Recuerda, Rocío
K1 Agua - Purificación
AB Membrane processes such as, reverse osmosis (RO), is one option for drinking water treatment where a highproduct quality is desired. RO membranes are able to effectively remove organic and inorganic compoundsand microorganisms from water. One of the most serious problems in this system is membrane fouling causedby the microbial biofilm formation. Biofilms contributes to RO membrane deterioration, but it also were associated with human health risks, since they can be reservoirs of human opportunistic pathogens and/or antibioticresistant bacteria. In the present study, samples from a drinking-water RO system were analyzed to determinethe bacterial composition of the different frames of RO system: feed water, rejection water and permeatewater. A total of 215 strains were identified using two methodologies, one genomic (16S rRNA gene) andother proteomic (MALDI-TOF MS). The majority bacterial groups identified were Gammaproteobacteria,Firmicutes and Betaproteobacteria, whereas Alfaproteobacteria, Bacteroidetes, and Actinomycetales were the minority bacterial identified groups. Antibiotic resistance analysis of the bacterial strains to 14 antibiotics tested revealed that resistance to three or more classes of antibiotics was observed in 37.2% of the isolates, being theoccurrence of multiple antibiotic resistance phenotypes mainly related to the taxonomic affiliation of the microorganisms. Regarding of the bacterial groups, the antibiotic resistance percentages among the strains were 68.2%to β-lactams, 56.8% to macrolides, 36.4% to quinolones, 34.1% to cephalosporins, 22.7% to aminoglycosides and9.1% to tetracyclines. On the other hand, >60% of the bacterial strains produced biofilms in vitro; from them,Aeromonas caviae, Pseudomonas aeruginosa and Sphingopyxis terrae were strong biofilm formers
PB Elsevier
YR 2021
FD 2021-02-04
LK https://hdl.handle.net/10630/34082
UL https://hdl.handle.net/10630/34082
LA eng
NO Alejandro Labella, Rosana Molero, Rocío Leiva-Rebollo, Rocío Pérez-Recuerda, Juan J. Borrego, Identification, resistance to antibiotics and biofilm formation of bacterial strains isolated from a reverse osmosis system of a drinking water treatment plant, Science of The Total Environment, Volume 774, 2021, 145718, ISSN 0048-9697, https://doi.org/10.1016/j.scitotenv.2021.145718.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026