RT Journal Article
T1 In vitro T-cell responses to beta-lactam drugs in immediate and nonimmediate allergic reactions.
T2 T-cell responses to beta-lactam drugs.
A1 Luque, Inmaculada
A1 Leyva-Fernández, Laura
A1 Torres-Jaén, María Josefa
A1 Rosal, Manuel
A1 Mayorga Mayorga, Cristobalina
A1 Segura, José Manuel
A1 Blanca, Miguel
A1 Juárez, Carlos
K1 Alergia a los medicamentos
K1 Antibióticos - Efectos secundarios
AB Background: Beta-Lactam drugs may induce both cellular and humoral allergic reactions, and there is evidence that T cells play an important role in the pathogenesis of these reactions. The aim of this work was to assess the sensitivity and specificity of the lymphocyte transformation test (LTT) as an in vitro diagnostic tool, in patients with either an immediate or a nonimmediate reaction to penicillin G and/or amoxicillin. Methods: Fifty patients with a well-documented history of allergic reactions to beta-lactams (31 immediate and 19 nonimmediate) were studied by means of skin tests (prick and intradermal), radioallergosorbent test (RAST), and, when necessary, controlled administration of the drug. Twenty-eight healthy subjects with good tolerance to penicillins served as controls. LTT was performed in all subjects. Results: Skin tests were positive in 77.4% of the patients with immediate reactions and in 36.8% of those with nonimmediate reactions. The overall sensitivity of LTT in the allergic patients was 62%, but, when analyzed separately, sensitivity was 64.5% for the immediate group and 57.9% for the nonimmediate group. The LTT specificity was 92.8%. Conclusions: The LTT should be considered a useful in vitro diagnostic tool to identify subjects allergic to penicillins, especially patients with nonimmediate reactions where the LTT has a better diagnostic value than skin tests. Interestingly, positive T-cell proliferative responses can be observed 10 or more years after the occurrence of the reaction without further exposure to the drug.
PB Wiley
YR 2001
FD 2001
LK https://hdl.handle.net/10630/32378
UL https://hdl.handle.net/10630/32378
LA eng
NO Luque I, Leyva L, José Torres M, Rosal M, Mayorga C, Segura JM, Blanca M, Juárez C. In vitro T-cell responses to beta-lactam drugs in immediate and nonimmediate allergic reactions. Allergy. 2001 Jul;56(7):611-8
NO Política de acceso abierto tomada de: https://v2.sherpa.ac.uk/id/publication/3704?template=romeo
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026