RT Journal Article
T1 Geminiviruses Subvert Ubiquitination by Altering CSN-Mediated Derubylation of SCF E3 Ligase Complexes and Inhibit Jasmonate Signaling in Arabidopsis thaliana
T2 Geminiviruses Subvert Ubiquitination
A1 Lozano-Durán, Rosa
A1 Rosas-Díaz, Tábata Victoria
A1 Gusmaroli, Giuliana
A1 Pérez-Luna, Ana Isabel
A1 Taconnat, Ludivine
A1 Wang Deng, Xing
A1 Rodríguez-Bejarano, Eduardo
K1 Virus fitopatógenos
AB Viruses must create a suitable cell environment and elude defense mechanisms, which likely involves interactions with hostproteins and subsequent interference with or usurpation of cellularmachinery. Here, we describe a novel strategy used by plantDNA viruses (Geminiviruses) to redirect ubiquitination by interfering with the activity of the CSN (COP9 signalosome) complex.We show that geminiviral C2 protein interacts with CSN5, and its expression in transgenic plants compromises CSN activity onCUL1. Several responses regulated by the CUL1-based SCF ubiquitin E3 ligases (including responses to jasmonates, auxins,gibberellins, ethylene, and abscisic acid) are altered in these plants. Impairment of SCF function is confirmed by stabilization ofyellow fluorescent protein–GAI, a substrate of the SCFSLY1. Transcriptomic analysis of these transgenic plants highlights theresponse to jasmonates as the main SCF-dependent process affected by C2. Exogenous jasmonate treatment of Arabidopsisthaliana plants disrupts geminivirus infection, suggesting that the suppression of the jasmonate response might be crucial forinfection. Our findings suggest that C2 affects the activity of SCFs, most likely through interference with the CSN. As SCFs arekey regulators of many cellular processes, the capability of viruses to selectively interfere with or hijack the activity of thesecomplexes might define a novel and powerful strategy in viral infections.
PB Oxford Academy
YR 2011
FD 2011-03
LK https://hdl.handle.net/10630/34772
UL https://hdl.handle.net/10630/34772
LA eng
NO Rosa Lozano-Durán, Tabata Rosas-Díaz, Giuliana Gusmaroli, Ana P. Luna, Ludivine Taconnat, Xing Wang Deng, Eduardo R. Bejarano, Geminiviruses Subvert Ubiquitination by Altering CSN-Mediated Derubylation of SCF E3 Ligase Complexes and Inhibit Jasmonate Signaling in Arabidopsis thaliana    , The Plant Cell, Volume 23, Issue 3, March 2011, Pages 1014–1032, https://doi.org/10.1105/tpc.110.080267
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 24 ene 2026