RT Journal Article
T1 Proepicardial Origin of Developing Coronary Vessels
T2 Formación de vasos coronarios a partir del proepicardio
A1 Palmquist-Gomes, Paul
A1 Pérez-Pomares, José María
A1 Guadix-Domínguez, Juan Antonio
K1 Miocardio
K1 Endotelio
AB Coronary irrigation of the myocardium is essential for homeostasis in the adult heart, and embryonic developmental abnormalities in these blood vessels may result in congenital heart defects. Despite the clinical relevance of coronary arteries, the embryonic mechanisms regulating their formation are not known in detail. Recent studies suggest that the epicardial progenitors (proepicardial cells [EP]) make a critical contribution to coronary artery morphogenesis. Transplantation of proepicardial quail cells to chicken hearts (Figure A) can be used to trace the destination of the donor cells in chimeric embryos, as endothelial quail cells–but not chicken cells–are labeled with QH1 antibody (Figure B-E, red signal; Ao, aorta; LA, left arium; LV, left ventricle; PA, pulmonary artery; RA, right atrium; RV, right ventricle). Intravascular injection of fluorescein-conjugated Lens culinaris lectin (FITC-LC) labels all blood vessels connected with systemic circulation (Figure B-E, green signal). The ventral views (Figure B) and dorsal views (Figure D; VS, venous sinus) of a chimeric heart (7 days of incubation) show how donor EP-derived endothelial cells (QH1+) form coronary vessels in heart chambers but not at the arterial pole of the heart (Figure B and C, discontinuous line), and become incorporated into the endothelium of coronary vessels whether connected (FITC-LC+, arrowheads of Figure C and E) or not connected with systemic circulation (FITC-LC–) (Figure C and E, arrows). The FITC-LC+/QH1– vessels are not derived from the quail EP.
PB Elsevier
YR 2019
FD 2019-02
LK https://hdl.handle.net/10630/45419
UL https://hdl.handle.net/10630/45419
LA eng
NO Palmquist-Gomes P, Pérez-Pomares JM, Guadix JA. Proepicardial Origin of Developing Coronary Vessels. Rev Esp Cardiol (Engl Ed). 2019 Feb;72(2):163. English, Spanish. doi: 10.1016/j.rec.2018.01.028. Epub 2018 May 4. PMID: 29735303.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 2 mar 2026