RT Conference Proceedings
T1 Characterization of the cyanobacterium Oscillatoria sp. isolated from extreme sulphureous water from Los Baños de la Hedionda (S Spain)
A1 Martín-Clemente, Elena
A1 Melero-Jiménez, Ignacio José
A1 Reul, Andreas
A1 Hernández-López, Miguel
A1 Salvo-Tierra, Ángel Enrique
A1 Bañares-España, Elena
A1 García-Sánchez, María Jesús
A1 Flores-Moya, Antonio
K1 Cianobacterias
AB Los Baños de la Hedionda (Málaga, S Spain) is a natural sulphureous spa (150-200 µM sulphide). Although this high sulphide levels can affect the photosynthetic process, there are numerous photosynthetic microorganisms inhabiting the spa. Among them, we isolated a strain of the cyanobacterium Oscillatoria sp., a genus well known by its tolerance to sulphide.Objectives Firstly, to analyze the photosynthetic characteristics and growth rate of the isolated strain, as well as the effect of the presence of sulphide in both processes. Secondly,  to determine the limit of genetic adaptation of this strain to sulphide.Methods The resistance of the isolated strain to sulphide was studied by analyzing the effect of increasing sulphide levels (up to 1600 µM) on photosynthetic performance and growth. The limit of genetic adaptation was explored using an evolutionary experimental design named as ratchet protocol. This design allows discerning the maximum capacity of genetic adaptation of Oscillatoria sp. to the exposure of increasing doses of sulphideConclusions The strain showed maximum growth rates at 200 µM sulphide although reduced rates can be found up to 800 µM sulphide. A significant increase in resistance was achieved in all derived populations during the ratchet experiment (surviving at sulphide concentrations higher than 2 mM). Moreover, they showed different evolutionary potential to adapt to sulphide, depending on historical contingency.
YR 2017
FD 2017-09-15
LK http://hdl.handle.net/10630/14471
UL http://hdl.handle.net/10630/14471
LA eng
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.Spanish Ministry of Science and Innovation through CGL2014-53682-P project. Predoctoral State Grant from Scientific and Technical Research and Innovation Plan, Spanish Ministry of Economy, Industry and Competitiveness I+D+i ECC/1402/2013, 2015
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026