RT Conference Proceedings T1 Transcriptomics of ammonium nutrition in the conifer Pinus pinaster Aiton A1 Ortigosa Peña, Francisco A1 Lobato-Fernández, César A1 Valderrama-Martín, José Miguel A1 Ávila-Sáez, Concepción A1 Cánovas-Ramos, Francisco Miguel A1 Cañas-Pendón, Rafael Antonio K1 Nitrógeno -- Metabolismo K1 Coníferas K1 Amonio AB Nitrogen is an important element for all living beings because it is part of macromolecules as significantas nucleic acids or amino acids. For plants, it constitutes a limiting factor in their growth and development1due to their low natural availability in soils thus limiting primary production in ecosystems2.Conifers are a group of gymnosperm plants that form large forest extensions of vegetation, being the mainconstituents of forests in boreal ecosystems3 where ammonium is the main source of inorganic nitrogen4.Due to the characteristics of the soils in which conifers usually grow, these plants have developed a hightolerance to the presence of ammonium, which may constitute their main source of inorganic nitrogen5.The maritime pine (Pinus pinaster Aiton) is a conifer that has a wide distribution in the westernMediterranean area and has been widely used in reforestation, soil stabilization tasks and industrially. Inrecent years, maritime pine has been the subject of multiple omic studies that have resulted in theacquisition of important tools and resources6,7.The present work is focused on the analysis of the ammonium uptake and management efficiency, and itsrelationship with the biomass accumulation in maritime pine. For this purpose, several experiments havebeen developed in which pine seedlings have undergone different levels of ammonium nutrition, both in theshort and long term. As a result of short-term experiments, the characterization of transcriptomic responseto the process of ammonium nutrition (uptake and assimilation) is being studied at mRNA, lncRNA andmiRNA level in roots. In relation to long-term experiments, ten different provenances of maritime pineseedlings were treated with different ammonium levels and the biomass changes were measured. The resultsobtained suggest the existence a certain phenotypic plasticity grade for this conifer. YR 2019 FD 2019-07-10 LK https://hdl.handle.net/10630/17990 UL https://hdl.handle.net/10630/17990 LA spa NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.This project was supported by a grant form the Spanish Ministerio de Ciencia, Innovación y Universidades(MicroNUpE, BIO2015-73512-JIN; MINECO/AEI/FEDER, UE). FO was supported by a grant from theUniversidad de Málaga (Programa Operativo de Empleo Juvenil vía SNJG, UMAJI11, FEDER, FSE, Juntade Andalucía) and JMVM by a grant from the Spanish Ministerio de Educación y Formación Profesional(FPU17/03517) DS RIUMA. Repositorio Institucional de la Universidad de Málaga RD 19 ene 2026