RT Journal Article
T1 Counterintuitive Fluorescence Blue Shift in Symmetry Breaking Dicationic Bis(indolium) with Two-Photon Absorption Properties for NIR Living Cell Imaging
A1 Benítez-Martín, Carlos
A1 Marín-Beloqui, José Manuel
A1 López Navarrete, Juan T.
A1 Casado-Cordón, Juan
A1 Nájera-Albendín, Francisco
A1 Perez-Inestrosa, Ezequiel
K1 Fluorescencia
K1 Mitocondrias
K1 Fotones - Absorción
AB Two-photon absorption (2PA) has become a cornerstone for bioimaging,enabling deeper tissue penetration while minimizing photodamage. Recently,the correlation between 2PA and excited-state symmetry breaking (ES-SB) hasbeen probed. Typically, the first hallmark of ES-SB is a red shift in fluorescencewith increasing solvent polarity. Herein, water-soluble bis(indolium)dyes, synthesized in only one step are reported, that exhibit remarkable2PA performance, coupled to the ES-SB process, displaying a counterintuitivefluorescence blue shift in polar solvents. The relationship betweentheir chemical structure and the extent of the ES-SB and 2PA properties isinvestigated via steady-state optical spectroscopies and transient absorptionspectroscopy (TAS). The unusual blue shift is ascribed to their dicationic nature,leading to an uneven stabilization of the ground and ES-SB excited statesin polar environments due to inefficient excited state solvation and positivecharge confinement. These compounds also offer specific mitochondrialstaining of living HeLa cells, performing at the level of more complexcommercial mitochondria markers. Herein, a novel family of dicationic dyesare introduced with robust 2PA properties and practical use in bioimagingand provide connections between their dication quadrupolar nature, whichimparts larger water solubility, 2PA performance, and ES-SB, which improvingfluorescence properties and bioimaging microscopy detection and resolution.
PB Wiley
YR 2025
FD 2025
LK https://hdl.handle.net/10630/40043
UL https://hdl.handle.net/10630/40043
LA eng
NO C. Benitez-Martin, J. M. Marin-Beloqui, J. T. L. Navarrete, J. Casado, F. Najera, and E. Perez-Inestrosa, “ Counterintuitive Fluorescence Blue Shift in Symmetry Breaking Dicationic Bis(indolium) with Two-Photon Absorption Properties for NIR Living Cell Imaging.” Adv. Mater. (2025): e10730. https://doi.org/10.1002/adma.202510730
NO Universidad de Málaga
NO Junta de Andalucía
NO Ministerio de Universidades
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 25 ene 2026