RT Journal Article
T1 Immune gene expression in gilthead seabream (Sparus aurata) after Lymphocystis disease virus (LCDV‐Sa) challenge resulting in asymptomatic infection
A1 Leiva Rebollo, Rocío
A1 Labella Vera, Alejandro Manuel
A1 Borrego-García, Juan José
A1 Castro-López, María Dolores
K1 Inhibidores víricos
AB Aims: To determine the immune gene expression response of giltheadseabream (Sparus aurata) that is experimentally infected with thelymphocystivirus LCDV-Sa.Methods and Results: Viral DNA and transcripts were detected by qPCR in allsamples from fish injected with LCDV-Sa, demonstrating that the virusestablish a systemic and asymptomatic infection. The expression of 23immune-related genes was also analysed by RT-qPCR in the head kidney (HK)and intestine at several times post-infection (dpi). In HK, the expression offive type I interferon (IFN)-related genes (ifn, irf3, mx2, mx3 and isg15), il10and ck10 was upregulated at 1–3 dpi, while genes related to the inflammationprocess (tnfa, il1ß, il6, casp1) were not differentially expressed or evendownregulated. The expression profile in the intestine was different regardingtype I INF-related genes. An upregulated c3 and ighm expression was observedin both HK and intestine at 3–8 dpi. Finally, the transcription of nccrp1 andmhcIIa was induced in HK, whereas tcrb expression was downregulated inboth organs.Conclusions: LCDV-Sa seems to trigger an immune response in giltheadseabream characterized by a partial activation of type I IFN system and a lackof systemic inflammatory response which may be related to viral persistence.Significance and Impact of the Study: The immune response observed ingilthead seabream infected by LCDV-Sa could be implicated in theestablishment of an asymptomatic persistent infection.
PB Oxford Academic
YR 2020
FD 2020-01-01
LK https://hdl.handle.net/10630/34081
UL https://hdl.handle.net/10630/34081
LA eng
NO R. Leiva‐Rebollo, A.M. Labella, J.J. Borrego, D. Castro, Immune gene expression in gilthead seabream (Sparus aurata) after Lymphocystis disease virus (LCDV‐Sa) challenge resulting in asymptomatic infection, Journal of Applied Microbiology, Volume 128, Issue 1, 1 January 2020, Pages 41–53, https://doi.org/10.1111/jam.14454
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026