RT Conference Proceedings
T1 Bioinformatics analysis of Pinus pinaster Aiton transcriptome in response to ammoniacal nutrition
A1 Lobato-Fernández, César
K1 Bioinformática
K1 Biología molecular
AB Conifers have a great economic and environmental value. Nitrogen is an important limiting factor for thedevelopment of plants. Conifers are well adapted to ammonium nutrition since it is the main source ofinorganic nitrogen in the ecosystems that they live.The present work is based on the study of ammonium uptake and management by maritime pine (Pinuspinaster Aiton) and its implication in the production of plant biomass. This conifer is present in westernMediterranean area and it is used for reforestation soil stabilization and industry. Due to its importancein this region maritime pine has been used as model conifer tree which has led to different omics studiesand resources.For this purpose we have developed several experiments using pine seedlings subjected to differentlevels of ammonium at the short and long term using the latest biotechnological and bioinformaticadvances. The studies of RNA-seq allow us to obtain a large amount of transcriptomic information ofmRNA lncRNA and miRNA level in the roots under ammonium nutrition. These data need differentbioinformatic workflows to extract expression results with reliability and biological context. Parallel tothese studies we have performed direct RNA sequencing using the Minion device of Oxford NanoporeTechnologies (ONT). Thanks to this kind of technology we have been able to identify epitranscriptomicschanges in our samples under ammonium nutrition. With this aim we have had to develop a newbioinformatic workflow. The characteristics of this new kind of sequencing allow us to analyze the RNAseq studies from another perspective.
YR 2019
FD 2019-07-30
LK https://hdl.handle.net/10630/18182
UL https://hdl.handle.net/10630/18182
LA eng
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026