RT Journal Article
T1 Dietary Administration of Postbiotics from Vibrio proteolyticus DCF12.2 Enhanced Intestinal Integrity, Microbiota, and Immune Response in Juvenile Gilthead Seabream (Sparus aurata)
A1 Pérez-Gómez, Olivia
A1 Rohra-Benítez, Sonia
A1 Domínguez-Maqueda, Marta
A1 Cerezo, Isabel M.
A1 Galafat, Alba
A1 Martínez-Manzanares, Eduardo
A1 Mancera, Juan Miguel
A1 Alarcón-López, Franciasco Javier
A1 García-Márquez, Jorge
A1 Moriñigo, Miguel Ángel
A1 Arijo, Salvador
K1 Doradas - Alimentación
K1 Peces - Alimentación
K1 Piscicultura
K1 Doradas - Inmunología
AB The use of postbiotics for dietary fortification in aquaculture is gaining increasing attention due to their potential immunomodulatory and gut health benefits. In this study, we evaluated the effects of postbiotics derived from Vibrio proteolyticus DCF12.2 on intestinal histology, microbiota composition, and the expression of genes related to epithelial integrity and inflammation in juvenile gilthead seabream (Sparus aurata). Fish were fed either a control (CRTL) diet or the postbiotic-supplemented diet (VP) for 62 days. At the end of the feeding trial, a lipopolysaccharide (LPS) challenge was conducted to evaluate the immune response in fish. Histological analysis revealed a healthy mucosa in both groups, though fish fed the VP diet reduced fold height and mucosal layer thickness, alongside a significant increase in goblet cells. Microbiota profiling indicated higher alpha diversity and significant shifts in community composition in the VP group, including enrichment of potentially beneficial genera (Pseudomonas, Sphingomonas) and depletion of opportunistic taxa (Enterococcus, Stenotrophomonas). After the feeding trial, fish fed the VP diet exhibited downregulation of pro-inflammatory markers (tnfα, cox2). Following LPS challenge, cdh1—a key epithelial adhesion protein required for maintaining intestinal barrier integrity—expression was upregulated significantly in the VP group, suggesting enhanced epithelial resilience. These findings demonstrate that dietary fortification with V. proteolyticus-derived postbiotics supports mucosal health as well as modulates the intestinal microbiota and immune responses in gilthead seabream juveniles, offering a promising strategy for functional aquafeed development in sustainable aquaculture.
PB MDPI
YR 2025
FD 2025-07-05
LK https://hdl.handle.net/10630/39320
UL https://hdl.handle.net/10630/39320
LA eng
NO Pérez-Gómez, O.; Rohra-Benítez, S.; Domínguez- Maqueda, M.; Cerezo, I.M.; Galafat, A.; Martínez-Manzanares, E.; Mancera, J.M.; Alarcón-López, F.J.; García-Márquez, J.; Moriñigo, M.Á.; et al. Dietary Administration of Postbiotics from Vibrio proteolyticus DCF12.2 Enhanced Intestinal Integrity, Microbiota, and Immune Response in Juvenile Gilthead Seabream (Sparus aurata). Animals 2025, 15, 1982. https:// doi.org/10.3390/ani15131982
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026