RT Journal Article
T1 Recognition of multiepitope dendrimeric antigens by human immunoglobulin E.
A1 Montañez-Vega, María Isabel
A1 Nájera-Albendín, Francisco
A1 Mayorga Mayorga, Cristobalina
A1 Ruiz-Sánchez, Antonio Jesús
A1 Vida-Pol, Yolanda
A1 Collado-Martín, Daniel
A1 Blanca, Miguel
A1 Torres-Jaén, María Josefa
A1 Pérez-de-Inestrosa-Villatoro, Ezequiel
K1 Inmunoquímica
K1 Dendrímeros
K1 Antígenos
AB In vitro drug allergy tests have limited sensitivity, partly due to a poor understanding of the immunological recognition of in vitro drug–protein conjugates. We have designed and synthesized multivalent mono- and bi-epitope dendrimeric antigen (DeAn) conjugates and studied their chemical and tridimensional structures. We describe differences in the spatial distribution and conformation of these conjugated epitopes for the first time: a partially hidden benzylpenicilloyl and a more exposed amoxicilloyl. Our data suggest that DeAn conjugates provide a useful model for studying IgE recognition in patients who suffer from an allergic reaction to benzylpenicillin and/or amoxicillin. 1D and 2D NMR, MDS and immunochemical studies provide evidence that both antigen composition and tridimensional distribution play key roles in IgE-antigen recognition. Bi-epitope DeAn conjugates could potentially allow the diagnosis of patients allergic to any of these two drugs with a single test and represent the basis for a broadly-applicable in vitro assay.
PB Elsevier
YR 2015
FD 2015-02-07
LK https://hdl.handle.net/10630/37574
UL https://hdl.handle.net/10630/37574
LA eng
NO Montañez, M. I., Najera, F., Mayorga, C., Ruiz-Sanchez, A. J., Vida, Y., Collado, D., Blanca, M., Torres, M. J., & Perez-Inestrosa, E. (2015). Recognition of multiepitope dendrimeric antigens by human immunoglobulin E. Nanomedicine: Nanotechnology, Biology and Medicine, 11(3), 579–588.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 22 ene 2026