RT Journal Article
T1 Antioxidant and Cytotoxic Effects on Tumor Cells of Exopolysaccharides from Tetraselmis suecica (Kylin) Butcher Grown Under Autotrophic and Heterotrophic Conditions
A1 Parra-Riofrío, Geovanna
A1 García-Márquez, Jorge
A1 Casas-Arrojo, Virginia
A1 Uribe-Tapia, Eduardo
A1 Abdala-Díaz, Roberto Teófilo
K1 Algas marinas - Aspectos moleculares
K1 Exopolisacáridos microbianos
AB Marine microalgae produce extracellular metabolites such as exopolysaccharides (EPS)with potentially beneficial biological applications to human health, especially antioxidant andantitumor properties, which can be increased with changes in crop trophic conditions. This studyaimed to develop the autotrophic and heterotrophic culture of Tetraselmis suecica (Kylin) Butcherin order to increase EPS production and to characterize its antioxidant activity and cytotoxiceffects on tumor cells. The adaptation of autotrophic to heterotrophic culture was carried outby progressively reducing the photoperiod and adding glucose. EPS extraction and purificationwere performed. EPS were characterized by Fourier-transform infrared spectroscopy and gaschromatography-mass spectrometry. The antioxidant capacity of EPS was analyzed by the2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) method, and the antitumor capacitywas measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay,showing high activity on human leukemia, breast and lung cancer cell lines. Although totalEPS showed no cytotoxicity, acidic EPS showed cytotoxicity over the gingival fibroblast cellline. Heterotrophic culture has advantages over autotrophic, such as increasing EPS yield,higher antioxidant capacity of the EPS and, to the best of our knowledge, this is the first probe thatT. suecica EPS have cytotoxic effects on tumor cells; therefore, they could offer greater advantages aspossible natural nutraceuticals for the pharmaceutical industry.
PB MDPI
YR 2020
FD 2020
LK https://hdl.handle.net/10630/45064
UL https://hdl.handle.net/10630/45064
LA eng
NO Parra-Riofrío, G.; García-Márquez, J.; Casas-Arrojo, V.; Uribe-Tapia, E.; Abdala-Díaz, R.T. Antioxidant and Cytotoxic Effects on Tumor Cells of Exopolysaccharides from Tetraselmis suecica (Kylin) Butcher Grown Under Autotrophic and Heterotrophic Conditions. Mar. Drugs 2020, 18, 534. https://doi.org/10.3390/md18110534
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 12 abr 2026