RT Journal Article
T1 The Physcomitrium patens egg cell expresses several distinct epigenetic components and utilizes homologs of BONOBO genes for cell specification
A1 Sanchez-Vera, Victoria
A1 Landberg, Katarina
A1 Lopez-Obando, Mauricio
A1 Thelander, Mattias
A1 Lagercrantz, Ulf
A1 Muñoz-Viana, Rafael
A1 Schmidt, Anja
A1 Grossniklaus, Ueli
A1 Sundberg, Eva
K1 Briofitas
K1 Células germinales
K1 Óvulos (Botánica)
AB Although land plant germ cells have received much attention, knowledge about their speci-fication is still limited. We thus identified transcripts enriched in egg cells of the bryophyte model species Physcomitrium patens, compared the results with angiosperm egg cells, and selected important candidate genes for functional analysis.  We used laser-assisted microdissection to perform a cell-type-specific transcriptome analy-sis on egg cells for comparison with available expression profiles of vegetative tissues and male reproductive organs. We made reporter lines and knockout mutants of the two BONOBO (PbBNB) genes and studied their role in reproduction.  We observed an overlap in gene activity between bryophyte and angiosperm egg cells, but also clear differences. Strikingly, several processes that are male-germline specific in Arabidop-sis are active in the P. patens egg cell. Among those were the moss PbBNB genes, which con-trol proliferation and identity of both female and male germlines.  Pathways shared between male and female germlines were most likely present in the com-mon ancestors of land plants, besides sex-specifying factors. A set of genes may also be involved in the switches between the diploid and haploid moss generations. Nonangiosperm gene networks also contribute to the specification of the P. patens egg cell.
PB Wiley
YR 2022
FD 2022-02-17
LK https://hdl.handle.net/10630/34357
UL https://hdl.handle.net/10630/34357
LA eng
NO Sanchez-Vera, V., Landberg, K., Lopez-Obando, M., Thelander, M., Lagercrantz, U., Muñoz-Viana, R., Schmidt, A., Grossniklaus, U. and Sundberg, E. (2022), The Physcomitrium patens egg cell expresses several distinct epigenetic components and utilizes homologues of BONOBO genes for cell specification. New Phytol, 233: 2614-2628. https://doi.org/10.1111/nph.17938
NO This work was supported by grants from the Knut and Alice Wallenberg Foundation (KAW; 2012.0087 to ES), the Swedish Research Council (VR; 621-2014-4941; 2018-04068 to ES), the University of Zurich, and a grant from the ‘Staatssekretariat f€ur Bildung und Forschung’ in the framework of COST action FA0903 (to UG and AS).
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026