RT Conference Proceedings
T1 The probiotic strain Shewanella putrefaciens Pdp11 strongly modulates gene expression of the fish pathogen Vibrio harveyi
A1 Delgado-Martín, Belén
A1 Cerezo Ortega, Isabel M.
A1 Domínguez-Maqueda, Marta
A1 Bautista-Moreno, Rocío
A1 Claros-Díaz, Manuel Gonzalo
A1 Tapia-Paniagua, Silvana Teresa
A1 Balebona-Accino, María del Carmen
A1 Moriñigo-Gutiérrez, Miguel Ángel
K1 Peces -- agentes patógenos
K1 Microbiología marina
AB In this work, the interaction between the fish probiotic Shewanella putrefaciens Pdp11 and the fish pathogen V. harveyi was studied by RNA-seq to understand how SpPdp11 interferes with the pathogen through bioinformatics analysis. Three types of cultures were performed: SpPdp11 alone, V. harveyi alone and SpPdp11 and V. harveyi together. RNA was extracted and sequenced (paired end, 2x75 bp) at the Ultrasequencing Service of the University of Málaga using the Illumina NextSeqTM 550 platform. Raw reads were processed using a bioinformatic pipeline and a network analysis was performed for the most relevant functional enrichment results. The results suggest that the presence of SpPdp11 affects V. harveyi to a greater extent than V. harveyi affects SpPdp11. Considering that V. harveyi is a pathogenic strain and SpPdp11 is a probiotic strain, this may be positive for its probiotic capacity, as it not only maintains its functionality almost intact, but also produces a huge imbalance in that of V. harveyi
YR 2022
FD 2022
LK https://hdl.handle.net/10630/25186
UL https://hdl.handle.net/10630/25186
LA eng
NO This work was funded by project AG-2017-509 83370-C3-3-R (MINECO, Spain).
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026