RT Journal Article
T1 Adipose tissue inflammation and VDR expression and methylation in colorectal cancer
A1 Castellano-Castillo, Daniel
A1 Morcillo Espina, Sonsoles
A1 Clemente-Postigo, María Mercedes
A1 Crujeiras Martínez, Ana Belén
A1 Fernández-García, José Carlos
A1 Torres Sánchez, Esperanza
A1 Tinahones-Madueño, Francisco José
A1 Macías-González, Manuel
K1 Cáncer colorrectal
K1 Tejido adiposo
AB Background: Lack of vitamin D (VD) has been associated with colorectal cancer (CRC). VD has anti-inflammatory effects and regulates several cellular pathways by means of its receptor, including epigenetic modifications. Adipose tissue dysfunction has been related to low-grade inflammation, which is related to diseases like cancer. The aim of this study was to explore the relationship between serum 25-hydroxyvitamin D (25(OH)D), adipose tissue gene expression of VD receptor (VDR), pro-inflammatory markers, and the epigenetic factor DNA methyltransferase 3a (DNMT3A) as well as VDR promoter methylation in CRC. Methods: Blood and visceral adipose tissue from 57 CRC and 50 healthy control subjects werecollected.CRCsubjects had lower serum 25(OH)D levels and higher VDR gene expression, and these were negatively correlated in the CRC group. Results: Adipose tissue NFκB1, IL6,andIL1B gene expression were higher in the CRC subjects than in the control subjects. 25(OH)D correlated negatively with NFκB1 and CRP. In turn, CRP correlated positively with NFκB1, IL6, IL1B,andVDR gene expression as well as NFκB1 that correlated positively with IL6 and IL1B. DNMT3A mRNA was negatively correlated with serum 25(OH)D and positively correlated with VDR DNA methylation. VDR DNA methylation at position 4 had lower levels in the CRC group. Global NFκB1 methylation at dinucleotide 3 was lower in the CRC group. Conclusion: Our results suggest that adipose tissue may be a key factor in CRC development. The low 25(OH)D levels and high adipose tissue VDR expression in CRC may, at least in part, mediate this relationship by modifying adipose tissue DNA methylation and promoting inflammation.
PB BMC (Part of Springer Nature)
YR 2018
FD 2018
LK https://hdl.handle.net/10630/34055
UL https://hdl.handle.net/10630/34055
LA eng
NO Castellano-Castillo, D., Morcillo, S., Clemente-Postigo, M. et al. Adipose tissue inflammation and VDR expression and methylation in colorectal cancer. Clin Epigenet 10, 60 (2018). https://doi.org/10.1186/s13148-018-0493-0
NO This study was supported by “Centros de Investigación En Red” (CIBER, CB06/03/0018) of the “Instituto de Salud Carlos III” (ISCIII) and grants from ISCIII (PI11/01661, PI15/0114) and co-financed by the European Regional Development Fund (FEDER). DCC was the recipient of a FPU grant from Education Ministry, Madrid, Spain (13/04211). MMG was the recipient of the Nicolas Monardes Programme from the “Servicio Andaluz de Salud, Junta de Andalucia”, Spain (C-0029-2014).
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026