RT Conference Proceedings
T1 Characterizing SYT6, a lipid transfer protein at the secretory pathway.
A1 Moya-Barrientos, Miriam
A1 Huércano, Carolina
A1 Morello-López, Jorge
A1 Cardeñas Echevarría, Carlos
A1 Boutté, Yohann
A1 Sánchez-Vera, Victoria
A1 Ruiz-López, Noemí
K1 Arabidopsis thaliana
AB The SYT6 protein from A. thaliana has recently been identified as a lipid transfer protein localized at membrane contact sites (MCS). MCS are regions where membranes of two organelles closely approach without membrane fusing. Historically, research has focused on endoplasmic reticulum (ER) and plasma membrane MCS, but recently MCS involving the ER and other organelles have come to light. SYT6 is a plant-exclusive protein exhibiting a modular structure shared with mammalian Extended-Synaptotagmins and other plant synaptotagmins, such as SYT1.  Our ongoing experiments suggest that SYT6 anchors itself to the ER via its transmembrane domain, contains a lipid trafficking domain (named SMP) and attaches to specific trans-Golgi Network (TGN) vesicles through its C2 domains and coiled-coil domain. These observations make SYT6 particularly intriguing, as its physiological roles remain unclear.  Currently, our focus is on studying SYT6 to uncover its expression, subcellular localization and most importantly, its function. Confocal imaging, has confirmed SYT6 attachment to the ER and to vesicles in continuous motion, suggesting involvement in secretory trafficking. Co-Immunoprecipitation and BiFC experiments, have confirmed the interaction between SYT6 and specific TGN proteins linked to the independent Golgi TGN (GI-TGN). Preliminary findings indicate a correlation between SYT6, and exocytosis. Furthermore, syt6 mutant displays altered negative gravitropism. Altogether, these findings suggest that SYT6 represents a novel ER-TGN CS protein that may play a role in secretory trafficking.
YR 2024
FD 2024
LK https://hdl.handle.net/10630/35583
UL https://hdl.handle.net/10630/35583
LA eng
NO This work has been funded by grant PID2021-127649OB-I00 (by MCIN/AEI/ 10.13039/501100011033 and by the European Union), Ayuda D2 Plan Propio by Universidad de Málaga and Proyecto QUAL21 012 IHSM (Consejería de Universidad, Investigación e Innovación, Junta de Andalucía).
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 4 mar 2026