RT Journal Article
T1 NADH-GOGAT Overexpression Does Not Improve Maize (Zea mays L.) Performance Even When Pyramiding with NAD-IDH, GDH and GS.
A1 Cañas-Pendón, Rafael Antonio
A1 Yesbergenova-Cuny, Zhazira
A1 Belanger, Léo
A1 Rouster, Jacques
A1 Brulé, Lenaïg
A1 Gilard, Françoise
A1 Quilleré, Isabelle
A1 Sallaud, Christophe
A1 Hirel, Bertrand
K1 Biomasa vegetal
K1 Maíz - Mejora genética
AB Maize plants overexpressing NADH-GOGAT were produced in order to determine if boosting 2-Oxoglurate production used as a carbon skeleton for the biosynthesis of amino acids will improve plant biomass and kernel production. The NADH-GOGAT enzyme recycles glutamate and incorporates carbon skeletons into the ammonium assimilation pathway using the organic acid 2-Oxoglutarate as a substrate. Gene pyramiding was then conducted with NAD-IDH and NADH-GDH, two enzymes also involved in the synthesis of 2-Oxoglurate. NADH-GOGAT overexpression was detrimental for shoot biomass production but did not markedly affect kernel yield. Additional NAD-IDH and NADH-GDH activity did not improve plant performance. A decrease in kernel production was observed when NADH-GDH was pyramided to NADH-GOGAT and NAD-IDH. This decrease could not be restored even when additional cytosolic GS activity was present in the plants overexpressing the three enzymes producing 2-Oxoglutarate. Detailed leaf metabolic profiling of the different transgenic plants revealed that the NADH-GOGAT over-expressors were characterized by an accumulation of amino acids derived from glutamate and a decrease in the amount of carbohydrates further used to provide carbon skeletons for its synthesis. The study suggests that 2-Oxoglutarate synthesis is a key element acting at the interface of carbohydrate and amino acid metabolism and that its accumulation induces an imbalance of primary carbon and nitrogen metabolism that is detrimental for maize productivity.
PB MDPI
YR 2020
FD 2020
LK https://hdl.handle.net/10630/40422
UL https://hdl.handle.net/10630/40422
LA eng
NO Cañas RA, Yesbergenova-Cuny Z, Belanger L, Rouster J, Brulé L, Gilard F, Quilleré I, Sallaud C, Hirel B. NADH-GOGAT Overexpression Does Not Improve Maize (Zea mays L.) Performance Even When Pyramiding with NAD-IDH, GDH and GS. Plants. 2020; 9(2):130. https://doi.org/10.3390/plants9020130
NO This research was supported by the French Banque Public of Investment (Bpifrance) in the framework of the PISI program (Project Innovation Strategic Industry) and several seed companies and by the Institut National de la Recherche Agronomique and by a Plant Biotechnology Industry program for BIOGEMMA.
NO RAC was supported by a grant from the Spanish Ministerio de Economía y Competitividad (BIO2015-73512-JIN; MINECO/AEI/FEDER, UE)
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 12 abr 2026