RT Conference Proceedings
T1 From bacterial antagonism to co-existence: the chemical interplay and adaptative strategies between Pseudomonas and Bacillus
A1 Molina-Santiago, Carlos
A1 Vela-Corcía, David
A1 Petras, Daniel
A1 Díaz-Martínez, Luis
A1 Pérez-Lorente, Alicia
A1 Pearson, John R.
A1 Caraballo-Rodríguez, Andrés Mauricio
A1 Dorrestein, Pieter
A1 De-Vicente-Moreno, Antonio
A1 Romero-Hinojosa, Diego Francisco
K1 Bacillus (Bacteria)
K1 Pseudomonas
AB Bacterial communities are continuously adapting and evolving for survival. They produce and secrete a broad range of molecules that kill, defend, or mediate communication between cells of different lineages, thus shaping the final structure of the microbial community. In this work, with the combination of -omics approaches, molecular biology and microscopic techniques, we expand our knowledge on the chemical interplay and specific mutations that modulate the transition from antagonism to co-existence between two plant-beneficial bacteria, Pseudomonas chlororaphis and Bacillus amyloliquefaciens. We demonstrate that bacillaene, a secondary metabolite with bacteriostatic activity produced by Bacillus, interacts with the protein elongation factor FusA of P. chlororaphis to arrest its growth and population advancement. Point mutations in this protein lead to tolerance to bacillaene and other inhibitors of protein translation. Additionally, we describe the key role of the glycerol kinase GlpK from B. amyloliquefaciens in its unspecific tolerance against P. chlororaphis. Mutations in GlpK provoked by a decrease of Bacillus cell membrane permeability among other pleiotropic responses. We conclude that nutrient specialization and mutations in basic biological functions are bacterial adaptive dynamics that lead to the coexistence of two primary competitive bacterial species rather than their mutual eradication.
YR 2022
FD 2022
LK https://hdl.handle.net/10630/25119
UL https://hdl.handle.net/10630/25119
LA eng
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 15 abr 2026