RT Conference Proceedings
T1 Differences in the viral induction of the senegalese sole Mx protein in RTG-2 and CHSE-214 cells
A1 Álvarez-Torres, Daniel
A1 Podadera, Ana Maria
A1 García-Rosado, Esther
A1 Collet, Bertrand
A1 Béjar-Alvarado, Julia
A1 Alonso-Sánchez, María del Carmen
K1 Virología - Investigación
AB Transfected RTG-2 cells infected with VHSV showed significant induction of theluciferase reporter gene, compared to the control non-infected cells, at 24, 48 and72 h post infection (p.i.). The maximum expression was recorded at 72 h p.i. (2.25folds compared to the control cells). In these cells, the infection with IPNV andEHNV did not result in the luciferase expression at any time tested. In transfectedCHSE-214 cells, EHNV stimulated luciferase expression at 24 h p.i. (2.17 foldscompared to the control cells), whereas cells infected with IPNV and VHSV didnot show luciferase activity at any time. The lack of induction of the SsMxpromoter after VHSV infection in CHSE-214 cells, as well as after EHNVinfection in RTG-2 cells may be caused by viral IFN-suppression mechanisms, ashas been demonstrated for IPNV in previous studies. Furthermore, the differentinduction of the SsMx promoter observed in RTG-2 and CHSE-214 cells afterinfection with the same virus indicates that cellular specific factors are involved inthe IFN-signaling response. Therefore, the use of two different cellular systemsmight be an interesting approach to identify such cellular factors.
PB EAFP
YR 2013
FD 2013
LK http://hdl.handle.net/10630/6745
UL http://hdl.handle.net/10630/6745
LA eng
NO UNIVERSIDAD DE MÁLAGA. CAMPUS DE EXCELENCIA INTERNACIONAL ANDALUCÍA TECH
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 5 mar 2026