RT Conference Proceedings
T1 Adhesin proteins in biofilms of the food-borne pathogen Bacillus cereus
A1 Caro-Astorga, Joaquín
A1 Pérez-García, Alejandro
A1 Romero, Diego
A1 De-Vicente-Moreno, Antonio
K1 Biopelículas
K1 Bacillus cereus
AB One of the greatest challenges of the food industry is to reduce food poisoning caused by food-borne pathogenic bacteria. Bacillus cereus is one the recurrent bacterial pathogens responsible of many outbreaks. Biofilm formation and sporulation are thought as the most important reservoirs of B. cereus. Biofilms are bacterial communities difficult to eradicate from biotic and abiotic surfaces. They are usually composed of exopolysaccharides and proteins. In our study, we are interested in the characterization of the molecular bases leading to biofilm formation in B. cereus during the interaction with plants. To start this analysis we have focused on the structural components of the extracellular matrix of these biofilms. Using as a model the well-studied B. subtilis, we show in this work the presence in B. cereus of an orthologous of tasA. TasA forms amyloid-like fibers in the extracellular matrix of B. subtilis. We demonstrate that the heterologous expression of this allele in a B. subtilis strain lacking the entire tasA operon and unable to form biofilm, restores the assembly of biofilm formation. Biochemical and morphological studies of this protein let us think on a possible amyloid-like nature of this protein, which could be involved in cell-cell interaction and biofilm formation as it does in B. subtilis
YR 2014
FD 2014-07-28
LK http://hdl.handle.net/10630/7942
UL http://hdl.handle.net/10630/7942
LA eng
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026