RT Journal Article
T1 Reference genes for gene expression studies in the mouse heart
A1 Ruiz-Villalba, Adrián
A1 Mattiotti, Andrea
A1 Gunst, Quinn D
A1 Cano-Ballesteros, Sara
A1 Van, Maurice JB
A1 Ruijter, JM
K1 Corazón - Enfermedades
AB To be accurate, quantitative Polymerase Chain Reaction (qPCR) studies require a set of stable reference genes for normalization. This is especially critical in cardiac research because of the diversity of the clinical and experimental conditions in the field. We analyzed the stability of previously described as potential reference genes in different subsets of cardiac tissues, each representing a different field in cardiac research. The qPCR dataset was based on 119 different tissue samples derived from cardiac development to pathology in mouse adult hearts. These samples were grouped into 47 tissue types. The stability of 9 candidate genes was analyzed in each of 12 experimental conditions comprising different groupings of these tissue types. Expression stability was determined with the geNorm module of qbase+. This analysis showed that different sets of two or three reference genes are required for analysis of qPCR data in different experimental conditions in murine cardiac research.
PB Springer Nature
YR 2017
FD 2017
LK https://hdl.handle.net/10630/33655
UL https://hdl.handle.net/10630/33655
LA eng
NO Ruiz-Villalba, A., Mattiotti, A., Gunst, Q.D. et al. Reference genes for gene expression studies in the mouse heart. Sci Rep 7, 24 (2017). https://doi.org/10.1038/s41598-017-00043-9
NO CardioNeT, the EU FP7-Marie Curie-ITN actions ITN-GA-2011-289600 grant
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 25 ene 2026