RT Journal Article
T1 A putative novel type of tight adherence (tad) like gene cluster of Pseudomonas Chlororaphis PCL1606 exhibits a crucial role in avocado roots colonization, fostering its biological control activity
A1 Ruiz-Muñoz, Blanca
A1 Rodríguez-García, María
A1 Heredia Ponce, Zaira María
A1 Tienda, Sandra
A1 Villar-Moreno, Rafael
A1 Arrebola-Díez, Eva María
A1 De-Vicente-Moreno, Antonio
A1 Cazorla-López, Francisco Manuel
A1 Gutiérrez Barranquero, José Antonio
K1 Rizobacterias
K1 Agentes antifúngicos
AB AimsPseudomonas chlororaphis PCL1606 (PcPCL1606), displays strong antagonistic and biological control abilities against several soil-borne fungal pathogens mainly due to the production of the antifungal molecule 2-hexyl, 5-propyl resorcinol (HPR). HPR governs other beneficial phenotypes, suggesting its additional regulatory activity. Published transcriptomic data identifying HPR-regulated genes involved in the interaction of PcPCL1606 with the avocado rhizosphere were used as a target database to identify putative genes involved in avocado roots colonization.MethodsThe induction of several consecutive genes that showed homology with genes encoding a putative type IV Flp/Tad (tight adherence) pilus but with a few differences from the Tad type A and B was observed. To study the role of this tad-like gene cluster in the biology of PcPCL1606, a chromosomal deletion mutant was constructed. The molecular characterization of the tad-like gene cluster and different in vitro and in vivo phenotypes related to colonization were addressed in the mutant strain respect to PcPCL1606.ResultsThe tad-like gene cluster was composed of five independent transcriptional units. Furthermore, the tad-like deletion mutant was impaired in early attachment, early biofilm formation, bacterial autoggregation and in root competitiveness in avocado plants and biocontrol activity against R. necatrix.ConclusionsThese results expand our understanding about the role of HPR as a putative signalling molecule. This study revealed the importance of a putative novel type of a Tad system of PcPCL1606 in the avocado roots colonization, confirming that initial attachment to roots is a fundamental mechanism for the PcPCL1606 rhizospheric performance.
PB Springer Nature
YR 2025
FD 2025-01-22
LK https://hdl.handle.net/10630/36789
UL https://hdl.handle.net/10630/36789
LA eng
NO Ruiz-Muñoz, B., Rodríguez-García, M., Heredia-Ponce, Z. et al. A putative novel type of tight adherence (tad) like gene cluster of Pseudomonas chlororaphis PCL1606 exhibits a crucial role in avocado roots colonization, fostering its biological control activity. Plant Soil (2025). https://doi.org/10.1007/s11104-024-07200-w
NO Funding for open access publishing: Universidad de Málaga/CBUA .This work was supported by PID2021-123713OB-I00 research project from the Ministerio de Ciencia, Innovacion y Universidades (Spain) and UMA18-FEDERJA-046 grant from Junta de Andalucía, both partially supported by the European Union (FEDER).Funding for open access charge: Universidad de Málaga / CBUA
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 12 abr 2026