RT Journal Article
T1 Intramyocardial Sprouting Tip Cells Specify Coronary Arterialization.
A1 Cano-Rincón, Elena
A1 Schwarzkopf, Jennifer
A1 Kanda, Masatoshi
A1 Lindberg, Eric
A1 Hollfinger, Irene
A1 Pogontke Díaz, Cristina
A1 Braeuning, Caroline
A1 Fischer, Cornelius
A1 Hübner, Norbert
A1 Gerhardt, Holger
K1 Arterias coronarias
AB The elaborate patterning of coronary arteries critically supports the high metabolic activity of the beating heart. How coronary endothelial cells coordinate hierarchical vascular remodeling and achieve arteriovenous specification remains largely unknown. Understanding the molecular and cellular cues that pattern coronary arteries is crucial to develop innovative therapeutic strategies that restore functional perfusion within the ischemic heart.Results: We discover that coronary arteries originate from cells that have previously transitioned through a specific tip cell phenotype. We identify nonoverlapping intramyocardial and subepicardial tip cell populations with differential gene expression profiles and regulatory pathways. Esm1-lineage tracing confirmed that intramyocardial tip cells selectively contribute to coronary arteries and endocardial tunnels, but not veins. Notably, prearterial cells are detected from development stages to adulthood, increasingly in response to ischemic injury, and in human embryos, suggesting that tip cell-to-artery specification is a conserved mechanism.Conclusions: A tip cell-to-artery specification mechanism drives arterialization of the intramyocardial plexus and endocardial tunnels throughout life and is reactivated upon ischemic injury. Differential sprouting programs govern the formation and specification of the venous and arterial coronary plexus.
PB American Heart Association
YR 2024
FD 2024
LK https://hdl.handle.net/10630/34033
UL https://hdl.handle.net/10630/34033
LA eng
NO This project was supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation, SFB1366—B06 and SFB1470—A04) and by the Deutsches Zentrum für Herz-Kreislauf-Forschung (DZHK; German Center for Cardiovascular Research). E. Cano was also supported by a Postdoctoral Fellowship from Ramon Areces Foundation.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026