RT Conference Proceedings
T1 FaRIF Transcription Factor Plays a Key Role in the Regulation of Fruit Ripening in the Cultivated Strawberry Fragaria x ananassa
A1 Martín-Pizarro, Carmen
A1 Meco, Victoriano
A1 Vallarino Castro, José G.
A1 Pillet, Jeremy
A1 Casañal, Ana
A1 Merchante-Berg, Catharina
A1 Giovannoni, James J.
A1 Osorio-Algar, Sonia
A1 Botella-Mesa, Miguel Ángel
A1 Posé-Padilla, David
A1 Valpuesta-Fernández, Victoriano
K1 Fresa
AB Strawberry is becoming a model for studying the molecular mechanism of ripening in non-climacteric fruits. However, a limited number of transcriptional regulators of this process have been identified so far. In this study, we have identified and characterized a gene encoding for a NAC transcription factor (TF), named as FaRIF (Ripening Inducing Factor). FaRIF expression presents a fruit-specific pattern, which is upregulated during ripening. In order to functionally characterize this TF, we have generated silencing (35S::RIF-RNAi) and overexpressing (35S::RIF-GFP) stable transgenic lines. While the RNAi lines showed an apparent delay of fruit ripening, the overexpressing lines displayed an acceleration of this process. Transcriptomic analysis, by RNA-seq, of the silenced lines showed a significantly altered expression of genes involved in the flavonoids pathway, as well as genes of the metabolism of the main sugars of the fruit. Metabolomics analysis confirmed these changes in the transgenic fruits. Both, transcriptomic and metabolomics data, were in agreement with the general phenotype observed in the fruits of the FaRIF-silenced lines. All together, our results support a main role of FaRIF in the control of relevant ripening-associated processes in strawberry fruit.
YR 2018
FD 2018-07-12
LK https://hdl.handle.net/10630/16198
UL https://hdl.handle.net/10630/16198
LA eng
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 4 mar 2026