RT Journal Article
T1 Rapid amplitude-modulation of a diarylethene photoswitch: En route to contrast-enhanced fluorescence imaging
A1 Naren, Gaowa
A1 Larsson, Wera
A1 Benítez-Martín, Carlos
A1 Li, Shiming
A1 Perez-Inestrosa, Ezequiel
A1 Albinsson, Bo
A1 Andréasson, Joakim
K1 Fluorescencia
AB A water soluble diarylethene (DAE) derivative that displays exceptionally intense fluorescence from thecolorless open form has been synthesized and characterized using UV/vis spectroscopy andfluorescence microscopy. We show that the bright emission from the open form can be rapidly switchedusing amplitude modulated red light, that is, by light at wavelengths longer than those absorbed by thefluorescent species. This is highly appealing in any context where undesired background fluorescencedisturbs the measurement, e.g., the autofluorescence commonly observed in fluorescence microscopy.We show that this scheme is conveniently applicable using lock-in detection, and that robust amplitudemodulation of the probe fluorescence is indeed possible also in cell studies using fluorescence microscopy
PB The Royal Society of Chemistry
YR 2021
FD 2021
LK https://hdl.handle.net/10630/36101
UL https://hdl.handle.net/10630/36101
LA eng
NO Rapid amplitude-modulation of a diarylethene photoswitch: En route to contrast-enhanced fluorescence imaging Gaowa Naren, Wera Larsson, Carlos Benitez-Martin, Shiming Li, Ezequiel Perez-Inestrosa, Bo Albinsson and Joakim Andréasson Chemical Science, 2021, 12, 7073-7078. DOI: 10.1039/D1SC01071A
NO Swedish Research Council VRSpanish Ministry for Science, Innovation, and UniversitiesUniversidad de Málaga-Junta de AndalucíaFEDER
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 4 mar 2026