RT Journal Article
T1 Differential iNKT and T cells activation in non-alcoholic fatty liver disease and drug-induced liver injury.
A1 Caballano-Infantes, Estefanía
A1 García-García, Alberto
A1 Lopez-Gomez, Carlos
A1 Cueto-Sánchez, Alejandro
A1 Robles-Díaz, María Mercedes
A1 Ortega-Alonso, Aida
A1 Martín-Reyes, Flores
A1 Álvarez-Álvarez, Ismael
A1 Arranz-Salas, Isabel María
A1 Ruiz-Cabello, Francisco
A1 Lucena-González, María Isabel
A1 García-Fuentes, Eduardo
A1 Andrade-Bellido, Raúl Jesús
A1 García-Cortés, Miren
K1 Hígado - Enfermedades
K1 Hígado - Fibrosis
K1 Inmunohematología
K1 Efectos indeseables de los medicamentos
AB Background: Non-alcoholic fatty liver disease (NAFLD) and idiosyncratic drug-induced liver injury (DILI) could share molecular mechanisms involving the immune system. We aimed to identify activation immunological biomarkers in invariant natural killer T (iNKT) and CD4/CD8+ T cells in NAFLD and DILI.Methods: We analyzed the activation profile (CD69, CD25, and HLA-DR) and natural killer group 2 member D (NKG2D) on iNKT cells, and CD4/CD8 T cells in peripheral blood mononuclear cells from NAFLD, with or without significant liver fibrosis, and DILI patients.Results: There was an increase in iNKT cells in NAFLD patients compared to DILI or control subjects. Regarding the cellular activation profile, NAFLD with significant liver fibrosis (F ≥ 2) displayed higher levels of CD69+iNKT cells compared to NAFLD with none or mild liver fibrosis (F ≥ 1) and control patients. CD69+iNKT positively correlated with insulin resistance, aspartate aminotransferase (AST) level, liver fibrosis-4 index (FIB4) and AST to Platelet Ratio Index (APRI). DILI patients showed an increase in CD69+ and HLA-DR+ in both CD4+ and CD8+ T cells, detecting the most relevant difference in the case of CD69+CD8+ T cells.Conclusions: CD69+iNKT may be a biomarker to assess liver fibrosis progression in NAFLD. CD69+CD8+ T cells were identified as a potential distinctive biomarker for distinguishing DILI from NAFLD.
PB MDPI
YR 2021
FD 2021-01-28
LK https://hdl.handle.net/10630/37970
UL https://hdl.handle.net/10630/37970
LA eng
NO Caballano-Infantes E, García-García A, Lopez-Gomez C, Cueto A, Robles-Diaz M, Ortega-Alonso A, Martín-Reyes F, Alvarez-Alvarez I, Arranz-Salas I, Ruiz-Cabello F, Lucena IM, García-Fuentes E, Andrade RJ, García-Cortes M. Differential iNKT and T cells activation in non-alcoholic fatty liver disease and drug-induced liver injury. Biomedicines. 2021;10(1):55
NO This work was supported in part by a grant from the Instituto de Salud Carlos III (Spain) (PI18/01804, PI19/00883, PI21/01248), from the Consejería de Economía, Conocimiento, Empresas y Universidad (Junta de Andalucía, Spain) (UMA18-FEDERJA-194, PI18-RT-3364), and from the Consejería de Salud (Junta de Andalucía, Spain) (PI-0285-2016). This study has been co-funded by FEDER funds (“A way to make Europe”) (“Andalucía se mueve con Europa”).
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026