RT Journal Article
T1 Factors affecting maturation of avocado somatic embryos
T2 Factores que afectan la maduración de embriones somáticos de aguacate
A1 Perán-Quesada, Rosa Amelia
A1 Sánchez-Romero, Carolina
A1 Barcelo-Muñoz, Araceli
A1 Pliego-Alfaro, Fernando
K1 Aguacates
AB The effect of mineral salts, sucrose, gellan gum, abscisic acid and coconut water on maturation of avocado (Persea americana Mill.) somatic embryos was studied. Use of B5 major salts was essential to obtain white-opaque embryos. Sucrose at 175 mM, gellan gum (6.8 g l−1 ) or coconut water (10–20%) also enhanced the recovery of white-opaque embryos. Abscisic acid slightly enhanced the appearance of white-opaque embryos. White-opaque embryos were further grown on MS, modified B5 mediumsupplemented with 10% coconut water or Jensen–MS media and they subsequently germinated on M1 medium. Higher germination rates (11.11%) were obtained with embryos that matured on modified B5 medium supplemented with 10% coconut water, although larger embryos developed on MS medium. Thus, embryo size during maturation does not seem to be correlated with germination capacity. Shoots obtained from germinated embryos could be multiplied in the presence of 4.44  M benzylaminopurine and successfully rooted (80% rate) after a 3 days treatment with 4.92  M indole-3-butyric acid. Over90% of the rooted shoots survived acclimatization.
PB Elsevier
YR 2004
FD 2004
LK https://hdl.handle.net/10630/37337
UL https://hdl.handle.net/10630/37337
LA eng
NO R. Perán-Quesada, C. Sánchez-Romero, A. Barceló-Muñoz, F. Pliego-Alfaro, Factors affecting maturation of avocado somatic embryos, 2004. Scientia Horticulturae, Vol 102, Issue 1, Pp 61-73, ISSN 0304-4238. https://doi.org/10.1016/j.scienta.2003.12.003.
NO Según recoge la web de ELSEVIER: https://www.elsevier.com/about/policies-and-standards/sharingPreprint: Authors can share their preprint anywhere at any time and If accepted for publication, we encourage authors to link from the preprint to their formal publication via its Digital Object Identifier (DOI).
NO This work was supported by the Comisión Interministerial de Ciencia y Tecnología, Spain(Grant No. AGL 2000–2003, C03-03).
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 22 ene 2026