RT Journal Article
T1 A Turn-On Two-Photon Fluorescent Probe for Detecting Lysosomal Hydroxyl Radicals in Living Cells.
A1 Benítez-Martín, Carlos
A1 Guadix-Domínguez, Juan Antonio
A1 Pearson, John
A1 Nájera-Albendín, Francisco
A1 Pérez-Pomares, José María
A1 Perez-Inestrosa, Ezequiel
K1 Espectroscopia de fluorescencia
K1 Lisosomas
AB The detection of reactive oxygen species (ROS) in biological systems and its imaging is of great importance. Among them, the hydroxyl radical (radical dotOH) stands out as one of the most reactive, playing an important role in many physiological and pathological processes. Here we report a two-photon (TP) turn-on fluorescent probe with good selectivity and sensitivity towards radical dotOH. The molecular design of this naphthalene-indoline compound enables it to accurately target lysosomes in its non-fluorescent (“off”) state, where interaction with radical dotOH gives rise to the fluorescent (“on”) state and allows for the imaging of radical dotOH generation in living mouse embryonic fibroblast (MEF) cells. The present study provides an approach for improving our understanding of the role of radical dotOH in biological processes.
PB Elsevier
YR 2019
FD 2019
LK https://hdl.handle.net/10630/36104
UL https://hdl.handle.net/10630/36104
LA eng
NO https://openpolicyfinder.jisc.ac.uk/id/publication/16792
NO Ministerio de Economía, Industria y Competitividad de EspañaConsejería de Salud, Junta de AndalucíaFEDER founds
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 12 abr 2026