RT Journal Article
T1 Host Range Determinants of Pseudomonas savastanoi Pathovars of Woody Hosts Revealed by Comparative Genomics and Cross-Pathogenicity Tests.
A1 Moreno-Pérez, Alba
A1 Pintado Calvillo, Adrián
A1 Murillo, Jesús
A1 Caballo-Ponce, Eloy
A1 Tegli, Stefania
A1 Moretti, Chiaraluce
A1 Rodríguez-Palenzuela, Pablo
A1 Ramos-Rodríguez, Cayo Juan
K1 Pseudomonas - Genética
K1 Bacterias gram negativas
K1 Genómica
K1 Bacterias fitopatógenas
K1 Virulencia (Microbiología)
K1 Fitotoxinas
K1 Proteínas bacterianas
K1 Plantas huésped
AB The study of host range determinants within the Pseudomonas syringae complex is gaining renewed attention due to its widespread distribution in non-agricultural environments, evidence of large variability in intra-pathovar host range, and the emergence of new epidemic diseases. This requires the establishment of appropriate model pathosystems facilitating integration of phenotypic, genomic and evolutionary data. Pseudomonas savastanoi pv. savastanoi is a model pathogen of the olive tree, and here we report a closed genome of strain NCPPB 3335, plus draft genome sequences of three strains isolated from oleander (pv. nerii), ash (pv. fraxini) and broom plants (pv. retacarpa). We then conducted a comparative genomic analysis of these four new genomes plus 16 publicly available genomes, representing 20 strains of these four P. savastanoi pathovars of woody hosts. Despite overlapping host ranges, cross-pathogenicity tests using four plant hosts clearly separated these pathovars and lead to pathovar reassignment of two strains. Critically, these functional assays were pivotal to reconcile phylogeny with host range and to define pathovar-specific genes repertoires. We report a pan-genome of 7,953 ortholog gene families and a total of 45 type III secretion system effector genes, including 24 core genes, four genes exclusive of pv. retacarpa and several genes encoding pathovar-specific truncations
PB Frontiers
YR 2020
FD 2020-07-02
LK https://hdl.handle.net/10630/39000
UL https://hdl.handle.net/10630/39000
LA eng
NO Moreno-Pe´rez A, Pintado A, Murillo J, Caballo-Ponce E, Tegli S, Moretti C, Rodr´ıguez-Palenzuela P and Ramos C (2020) Host Range Determinants of Pseudomonas savastanoi Pathovars of Woody Hosts Revealed by Comparative Genomics and CrossPathogenicity Tests. Front. Plant Sci. 11:973. doi: 10.3389/fpls.2020.00973
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026