RT Journal Article
T1 The effect of type I collagen on osteochondrogenic differentiation in adipose-derived stromal cells in vivo.
A1 Alonso, Mónica
A1 Claros-Gil, Silvia
A1 Becerra-Ratia, José
A1 Andrades-Gómez, José Antonio
K1 Colágeno
K1 Células madre
AB BackgroundThe objective of this study was to investigate whether type I collagen would elicit in vivo bone formation of passaged rat adipose-derived adult stromal cells (ADASC) placed extraskeletally.MethodsAfter expansion for 1–4 passages (P), cells were incubated in osteogenic medium containing dexamethasone, ascorbic acid and β-glycerol phosphate for 2–4 weeks. Undifferentiated cells were maintained in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS). Osteogenic differentiation was evaluated by alkaline phosphatase (ALP) and von Kossa staining as well as by gene expression of ALP, osteopontin (OP), osteonectin (ON), osteocalcin (OC), collagen I (colI), collagen II (colII), bone sialoprotein (BSP), periostin (Postn), runx2, osterix (Osx), sox9, msx1 and msx2. Diffusion chambers were filled with 1 × 106 cells mixed with or without type I collagen gel and implanted subcutaneously into rats. Controls included chambers exposed to (1) undifferentiated cells (with or without collagen, (2) collagen without cells and (3) empty chambers (n=5 per group).ResultsFour weeks after implantation, in vivo bone and cartilage formation was demonstrated in implants containing 4-week osteo-induced P1 and P4 cells wrapped in the collagen gel, as confirmed by Goldner's trichrome and Alcian blue staining, respectively. Newly formed bone stained positive for type I collagen. Control implants had no bone or cartilage and were primarily filled with fibrous tissue at that time interval.DiscussionRecent studies have demonstrated that ADASC offer great promise for cell-based therapies because of their ability to differentiate toward bone, cartilage and fat. However, the influence of different matrices on the in vivo osteogenic capability of ADASC is not fully understood. These findings suggest that type I collagen may support the survival and expression of osteogenic and chondrogenic phenotypes in passaged rat ADASC in vivo.
PB Elsevier
YR 2008
FD 2008
LK https://hdl.handle.net/10630/33273
UL https://hdl.handle.net/10630/33273
LA eng
NO https://v2.sherpa.ac.uk/id/publication/27899
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026