RT Journal Article
T1 Functional Analysis of TM6 MADS-box gene in the Octoploid Strawberry by CRISPR/Cas9 directed mutagenesis
A1 Martín-Pizarro, Carmen
A1 Triviño, Juan Carlos
A1 Posé-Padilla, David
K1 Fresas
AB The B-class of MADS-box transcription factors has been studied in many plant species, but remains functionally unchar- acterized in Rosaceae. APETALA3 (AP3), a member of this class, controls petal and stamen identities in Arabidopsis. In this study, we identified two members of the AP3 lineage in cultivated strawberry, Fragaria × ananassa, namely FaAP3 and FaTM6. FaTM6, and not FaAP3, showed an expression pattern equivalent to that of AP3 in Arabidopsis. We used the CRISPR/Cas9 genome editing system for the first time in an octoploid species to characterize the function of TM6 in strawberry flower development. An analysis by high-throughput sequencing of the FaTM6 locus spanning the target sites showed highly efficient genome editing already present in the T0 generation. Phenotypic characterization of the mutant lines indicated that FaTM6 plays a key role in anther development in strawberry. Our results validate the use of the CRISPR/Cas9 system for gene functional analysis in F. × ananassa as an octoploid species, and offer new opportunities for engineering strawberry to improve traits of interest in breeding programs.
PB Oxford University Press
YR 2019
FD 2019-01
LK https://hdl.handle.net/10630/23593
UL https://hdl.handle.net/10630/23593
LA eng
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026