RT Journal Article
T1 Inhibition of Endothelial Inflammatory Response by HT-C6, a Hydroxytyrosol Alkyl Ether Derivative.
A1 Marrero Capitán, Ana Dácil
A1 Castilla, Laura
A1 Bernal, Manuel
A1 Manrique-Poyato, María Inmaculada
A1 Posligua-García, Joel
A1 Moya-Utrera, Federico
A1 Porras Alcalá, Cristina
A1 Espartero Sánchez, José Luis
A1 Sarabia-García, Francisco Ramón
A1 Rodríguez-Quesada, Ana María
A1 Medina-Torres, Miguel Ángel
A1 Martínez-Póveda, Beatriz Amparo
K1 Antiangiogénicos
K1 Arterias - Enfermedades
K1 Aceite de oliva
AB Hydroxytyrosol (HT) is a bioactive phenolic compound naturally present in olives andextra virgin olive oil (EVOO) which is described as an antioxidant, antitumoral and antiangiogenicmolecule. Previous studies of semi-synthetic HT-derivatives presented the hydroxytyrosyl alkyl etherHT-C6 as one of the most potent derivatives studied in the context of antioxidant, anti-platelet andantiangiogenic assays, but its direct effect on inflammation was not reported. In this work, we useRT-qPCR measure of gene expression, protein analysis by Western-blot and immunofluorescencetechniques, adhesion and migration functional assays and single-cell monitoring of reactive oxygenspecies (ROS) in order to explore in vitro the ability of HT-C6 to interfere in the inflammatory responseof endothelial cells (ECs). Our results showed that HT-C6 strongly reduces the TNF- -inducedexpression of vascular cell adhesion molecule 1 (VCAM1), intercellular cell adhesion molecule 1(ICAM1), E-selectin (SELE), C-C motif chemokine ligand 2 and 5 (CCL2 and CCL5) in HUVECs,impairing the chemotactic and adhesion potential of these cells towards THP-1 monocytes in vitro.In this work, we define a mechanism of action underlying the anti-inflammatory effect of HT-C6,which involves the abrogation of nuclear factor kappa B (NF- B) pathway activation in ECs. Theseresults, together with the ability of HT-C6 to reduce ROS formation in ECs, point to this compoundas a promising HT-derivative to be tested in the treatment of atherosclerosis.
PB MDPI
YR 2023
FD 2023-07-28
LK https://hdl.handle.net/10630/31607
UL https://hdl.handle.net/10630/31607
LA eng
NO Marrero, A. D., Castilla, L., Bernal, M., Manrique, I., Posligua-García, J. D., Moya-Utrera, F., Porras-Alcalá, C., Espartero, J. L., Sarabia, F., Quesada, A. R., Medina, M. Á., & Martínez-Poveda, B. (2023). Inhibition of Endothelial Inflammatory Response by HT-C6, a Hydroxytyrosol Alkyl Ether Derivative. Antioxidants, 12(8), 1513. https://doi.org/10.3390/antiox12081513
NO Partial funding for open access charge: Universidad de Málaga
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026