RT Journal Article
T1 Exercise-induced re-programming of age-related metabolic changes in microglia is accompanied by a reduction in senescent cells.
A1 Mela-Rivas, Virginia
A1 Mota, Bibiana C.
A1 Milner, Mark
A1 McGinley, Aoife
A1 Mills, Kingston H.G.
A1 Kelly, Aine M.
A1 Lynch, Marina Ann
K1 Ejercicio físico - Aspectos fisiológicos
K1 Sistema nervioso - Inflamación
K1 Glucólisis
K1 Fagocitosis
K1 Fosforilación oxidativa
K1 Neuroglia
AB contribute to age-related cognitive impairment. Exercise improves cognitive function in agedanimals, perhaps because of a modulatory effect on microglial activation. Recent evidenceindicates that inflammatory microglia are glycolytic, driven by an increase in 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), an enzyme that is describedas the master regulator of glycolysis. Here we investigated whether microglia from agedanimals exhibited a glycolytic signature and whether exercise exerted a modulatory effect onthis metabolic profile. Young (4 month-old) and aged (18 month-old) mice were trained for10 days on a treadmill. One day before sacrifice, animals were assessed in the novel objectrecognition and the object displacement tests. Animals were sacrificed after the last bout ofexercise, microglial cells were isolated, cultured for 5 days and assessed for metabolic profile.Performance in both behavioural tests was impaired in sedentary aged animals and exerciseattenuated this age-related effect. A significant increase in glycolysis, glycolytic capacity andPFKFB3 was observed in microglia from aged animals and exercise ameliorated these effects,while it also increased the phagocytic capacity of cells. The senescent markers, -galactosidaseand p16INK4A, were increased in microglia from sedentary aged mice, and expression of thesemarkers was significantly decreased by exercise. The data demonstrate that the exerciserelated improved cognition is orchestrated by a normalization of the metabolic profile andfunctionality of microglia.
PB Elsevier
YR 2020
FD 2020-07
LK https://hdl.handle.net/10630/40713
UL https://hdl.handle.net/10630/40713
LA eng
NO Mela V, Mota BC, Milner M, McGinley A, Mills KHG, Kelly ÁM, Lynch MA. Exercise-induced re-programming of age-related metabolic changes in microglia is accompanied by a reduction in senescent cells. Brain Behav Immun. 2020 Jul;87:413-428. doi: 10.1016/j.bbi.2020.01.012. Epub 2020 Jan 21. PMID: 31978523.
NO https://openpolicyfinder.jisc.ac.uk/id/publication/12478
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026