RT Journal Article
T1 Screening of Synergistic Interactions of Epigallocatechin-3-gallate with Antiangiogenic and Antitumor Compounds
A1 Rodríguez-Quesada, Ana María
A1 Medina-Torres, Miguel Ángel
A1 García-Vilas García, Javier Alejandro
K1 Cancer
AB Purpose: To screen for possible synergistic interactions of epigallocatechin-3-gallate(EGCG) with a selection of 10 anti-angiogenic or anti-tumor compounds on the survivalof endothelial and tumor cells.Methods: Human HMEC endothelial and MDA-MB231 breast cancer cells were treatedwith different concentrations of EGCG and the 10 tested compounds either as singleagents or in paired combinations with EGCG for 3 days and final survival of cells wasdetermined by the MTT assay. IC50 values, sensitization factors and combinationindexes were calculated.Results; IC50 values of 140±2 and 45±6 μM were determined for EGCG-treatedendothelial and tumor cells, respectively. IC50 values for all tested compounds werewithin the micromolar and the submillimolar range. The values of the sensitizationfactor increased and those of the combination index decreased for paired combinationsof EGCG with 4-methylumbelliferone. The opposite was true for the combination ofEGCG with vitamin D3. Other tested combinations did not exhibit a clear monotoniceffect but rather a biphasic behaviour.Conclusion: Combinations of EGCG and 4-methylumbelliferone synergisticallydecrease endothelial and tumor cell survival. In contrast, the presence of EGCGantagonizes with the antiproliferative effect exerted by vitamin D3 on endothelial andtumor cells.
PB Elsevier
YR 2016
FD 2016-07
LK http://hdl.handle.net/10630/14630
UL http://hdl.handle.net/10630/14630
LA eng
NO Synergy 3 (2016) 5-13
NO This is the accepted manuscript of the article that finally was published in Synergy with DOI: 10.1016/j.synres.2016.05.001
NO Supported by grants BIO2014-56092-R (MINECO and FEDER), P12-CTS-1507(Andalusian Government and FEDER) and funds from group BIO-267 (AndalusianGovernment). The "CIBER de Enfermedades Raras" is an initiative from the ISCIII(Spain). JAGV has the financial support of Vicerrectorado de Investigación yTransferencia (University of Málaga, Spain). The funders had no role in the studydesign, data collection and analysis, decision to publish or preparation of themanuscript.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 23 ene 2026