RT Conference Proceedings
T1 Bioinformatic analyses of olive pollen tube and seed transcriptomes and their integration in an interactive olive expression atlas
A1 Bullones, Amanda
A1 Fernández-Pozo, Noé
A1 Lima-Cabello, Elena
A1 Castro, Antonio Jesús
A1 Alché-Ramírez, Juan de Dios
A1 Claros-Díaz, Manuel Gonzalo
K1 Bioinformática
AB The olive tree (Olea europaea L.) is of great socioeconomic importance due to theproduction of oil and table olives, and the seed is also an emergent source ofnutraceuticals (Jiménez-Ruiz et al., 2020) and feed supplements (Maestri et al.,2019). Spain is the world's leading producer and, of the 272 varieties present in ourcountry, Picual is the most cultivated and the main source of olive oil. Despite itsrelevance, there are many unknown aspects in its biology. Here we study thedevelopment of the Picual pollen tube to discover the biological processes involvedand its potential regulation. Highly relevant transcripts like those of two RINGproteins and other two Zinc-finger containing proteins seem to indicate that theyplay a main role in the process of mitosis II, and its cross-talk with environmental anddevelopmental clues. In addition, Picual orthologues of the 14 olive allergens knownto date were determined and their expression in the pollen tube monitored.Allergens are consistently expressed during pollen tube development, most of themat a high level. Despite Ole e 1 being the first olive pollen allergen to be identified(Villalba et al., 1993), it is the second most abundant transcript, behind Ole e 14which was only recently described (Oeo-Santos et al., 2018), with Ole e 15 being thelast allergen identified (San Segundo-Acosta et al., 2019) and the one less expressed.We are characterizing the transcriptome of Picual seeds at different developmentalstages, where the biological processes of the endosperm that nourish the embryo,and the establishment of embryonic polarity are particularly overrepresented.Finally, an interactive gene expression atlas for olive tree (OliveAtlas) whereexpression data were mapped to the Picual reference genome and its gene modelannotation was created. This tool tries to ease the lack of bioinformatics andgenomics resources and assists olive research and breeding.
YR 2022
FD 2022
LK https://hdl.handle.net/10630/25666
UL https://hdl.handle.net/10630/25666
LA eng
NO P18-RT-1577RYC2020-030219-IUMA20-FEDERJA-029PID2020-113324GB-I00PID2021-125805OA-I00Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026