RT Conference Proceedings
T1 Bistable expression of relevant genes for Pseudomonas syringae virulence
A1 López-Pagán, Nieves
A1 Rufián Plaza, José Sebastián
A1 López-Márquez, Diego
A1 Ruiz-Albert, Francisco Javier
A1 Beuzón-López, Carmen del Rosario
K1 Biología - Congresos
AB BackgroundsHeterogeneity or phenotypic variation has been known to take place in microbial clonal populations for decades. Under certain regulatory circuits, heterogeneity in gene expression can be enhanced leading to a bimodal expression profile in homogeneous environments. This process is known as bistability. The relevance of these processes has been demonstrated in Salmonella enterica and other human pathogen in the establishment of antibiotic persistence, and it has been shown to affect virulence genes, and to be linked to the establishment of chronic persistence. Nevertheless, little is known about the occurrence or impact of these processes in the adaptation of bacteria to non-animal host.ObjectivesTo address the question of whether there is phenotypic heterogeneity in the expression of genes relevant for adaptation to a non-animal host of the model plant pathogen Pseudomonas syringae. MethodsTranscriptional chromosome-located fusion to reporter genes encoding fluorescent protein were constructed in P. syringae pv. phaseolicola. Genes selected were several encoding different elements of a type III secretion system (T3SS) and flagellin, since motility has been reported as counter-regulated with the T3SS, Expression from these genes was analyzed using single-cell analysis methods, such as flow cytometry and fluorescent microscopy.Conclusions We recently showed expression of T3SS genes is phenotypically heterogeneous in planta and becomes bistable under certain laboratory conditions through the action of a double regulatory loop on the transcriptional activator HrpL. We present here single cell analyses of the gene encoding flagellin, as well as its responses to different regulatory proteins.
YR 2017
FD 2017-09-06
LK http://hdl.handle.net/10630/14429
UL http://hdl.handle.net/10630/14429
LA eng
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026