RT Conference Proceedings
T1 Co-cultures between neurons and astrocytes to address Alzheimer´s disease pathology.
A1 Cáceres Palomo, Laura
A1 García-León, Juan Antonio
A1 Trujillo-Estrada, Laura Isabel
A1 López Oliva, Elba
A1 Vitorica Ferrández, Javier
A1 Gutiérrez-Pérez, Antonia
K1 Alzheimer, Enfermedad de - Patogénesis
AB Background: Alzheimer's disease (AD) is characterized by presenting a complex pathology, not fully resolved yet.This fact, together with the lack of reliable models, has impeded the development of effective therapies. Recently,several studies have shown that functional glial cell defects have a key role in the pathology of AD. However, thisglial dysfunction, currently, cannot be correctly modeled using the available animal models, so we hypothesizedthat cells derived from Alzheimer's patients can serve as a better platform for studying the disease. In this sense,human pluripotent stem cells (hPSC) allow the generation of different types of neural cells, which can be used fordisease modeling, identification of new targets and drugs development. Methods: We have a collection of hiPSCsderived from patients with sporadic forms of AD stratified based on APOE genotype. We have differentiated thesecells towards neural cells and mature them to neurons or astrocytes using a serum-free approach, to assessintrinsic differences between those derived from AD patients or healthy controls. Results: We have implemented aserum-free approach and generated neural precursors and astrocytes from all the lines tested. We observedifferences at the phenotypic level and a reduced capacity to differentiate towards neural lineage in those linesderived from APOE4 carriers. Conclusions: Our preliminary data suggest intrinsic differences in the neuraldifferentiation capacity between cell lines derived from APOE4 or APOE3 carrier subjects. Further experimentswould contribute to elucidate novel pathogenic pathways associated with neurodegeneration and susceptible oftherapeutic modulation, likely contributing to the development of new effective drugs against AD.
YR 2023
FD 2023
LK https://hdl.handle.net/10630/27518
UL https://hdl.handle.net/10630/27518
LA eng
NO This study wassupported by ISCiii (Spain), co-financed by FEDER funds, through grants PI21/00915 (AG) and PI21/00914 (JV);by Junta de Andalucia through Consejería de Economía and Conocimiento grants UMA20-FEDERJA-048 (JAGL),PY18-RT-2233 (to AG) and US-1262734 (JV), co-financed by Programa Operativo FEDER 2014-2020, andSNGJ4-11 (LCP).Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026