RT Conference Proceedings
T1 Silica Particle based nanocomposites for Specific IgE Determination to Betalactams
A1 Matamoros Castellano, Esther
A1 Gil-Ocaña, Violeta
A1 Jiménez, Isabel M.
A1 Mayorga Mayorga, Cristobalina
A1 Doña, Inmaculada
A1 Céspedes Lagos, José Antonio
A1 Montañez-Vega, María Isabel
A1 Vida-Pol, Yolanda
A1 Pérez-de-Inestrosa-Villatoro, Ezequiel
A1 Torres-Jaén, María Josefa
K1 Dendrímeros - Congresos
K1 Nanopartículas - Congresos
AB Current immunoassays for diagnosing betalactam (BL) allergy have limited sensitivity and poor specificity. These include ImmunoCAP, the commercial immunoassay, and the radioallergosorbent test (RAST), a handmade test performed only in specialized laboratories. Both are based on cellulose polymers modified with covalently attachedbetalactam-poly-L-lysine conjugates, which are able to interact with specific IgE (sIgE). In RAST, dendrimers have been used instead of poly-L-lysine in the formation of penicilloyl- conjugates, showing specific recognition by penicillin-sIgE. In this work, we propose the use of nanomaterials consisting on silica particles highly functionalized with drug-dendrimer conjugates (including mono- and bi-epitopic structures), as precise and controlled solid phases able to improve the tests’ reliability. The homogeneous composition of the nano-composites provides high reproducibility and quality, which is critical for hospital applications, while silica particles possess promising properties as biocompatibility, tuneable size, and functionalisation, and their preparation is affordable and reproducible.The use of nano-scaled silica particles improved the results of available immunoassays techniques in terms of sensitivity and specificity, providing the possibility of testing different betalactams, simultaneously.
YR 2023
FD 2023
LK https://hdl.handle.net/10630/28474
UL https://hdl.handle.net/10630/28474
LA eng
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026