RT Conference Proceedings
T1 Looking for specialized ribosomes in plants. Characterization of the riboprotein family L24.
A1 Duarte-Conde, José Antonio
A1 Sans-Coll, Gemma
A1 Merchante-Berg, Catharina
K1 Plantas - Mejora genética - Congresos
K1 Plantas - Adaptación - Congresos
AB Translation and its regulation play an important role in plant adaptation. Ribosomeshave traditionally been considered passive molecular players regarding which RNAto translate. However, this view is changing due to studies showing that specific andheterogeneous ribosomes can have an active role regulating the translation ofdifferent RNA subpools in mammals and bacteria (Genuth & Barna, 2019). In plants,the possibilities for specialization are much higher, as each ribosomal family isencoded by two to seven paralogs and there are several hints in the literaturepointing towards differential paralog roles. However, whether this heterogeneityprovides selective translation of specific mRNAs under particular cell conditions hasyet to be demonstrated.To address this question, we are characterizing two ribosomal families, RPL10 andRPL24, which contain three and two paralogs, respectively, and that are ubiquitouslyexpressed in Arabidopsis. Specific functions have been described for at least oneparalog of each family and paralog mutants show different phenotypes as well(Falcone Ferreyra et al., 2020; Zhou et al., 2010)We will provide evidence of phenotypic variance between paralog mutants in familiesRPL10 and RPL24 under control and abiotic stress conditions. To determine if thesephenotypes are due to different RNA populations being translationally affected ineach mutant, we have performed RNA-seq from total and polysomal RNA from WTand mutant plants. In addition, we are studying mutant complementation byRecombineering (Brumos et al, 2020), leveraging the system to exchange exonsbetween paralogs maintaining each other ́s regulatory elements therefore sheddinglight on whether they are functionally equivalent. We will present our progressregarding these objectives.
YR 2022
FD 2022-09-14
LK https://hdl.handle.net/10630/25146
UL https://hdl.handle.net/10630/25146
LA eng
NO Ministerio de Ciencia e Innovación, Ministerio de Economia, Industria y Competitividad y Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 3 mar 2026