RT Conference Proceedings
T1 Glia and neurons from human iPSCs to address the pathology of Alzheimer´s disease
A1 García-León, Juan Antonio
A1 Cáceres Palomo, Laura
A1 Trujillo Estrada, Laura
A1 López Oliva, Elba
A1 Vitorica Ferrández, Javier
A1 Gutiérrez-Pérez, Antonia
K1 Alzheimer, Enfermedad de
AB Alzheimer's disease (AD) is characterized by presenting a complex pathology, not fully resolved yet. Thisfact, together with the lack of reliable models, has impeded the development of effective therapies.Recently, several studies have shown that functional glial cell defects have a key role in the pathology ofAD. However, this glial dysfunction, currently, cannot be correctly modeled using the available animalmodels, so we hypothesized that cells derived from Alzheimer's patients can serve as a better platform forstudying the disease. In this sense, human pluripotent stem cells (hPSC) allow the generation of differenttypes of neural cells, which can be used for disease modeling, identification of new targets and drugsdevelopment.Methods:We have a collection of hiPSCs derived from patients with sporadic forms of AD stratified based on APOEgenotype. We have differentiated these cells towards neural cells and mature them to neurons orastrocytes using a serum-free approach, to assess intrinsic differences between those derived from ADpatients or healthy controls.Results:We have implemented a serum-free approach and generated neural precursors and astrocytes from all thelines tested. We observe differences at the phenotypic level and a reduced capacity to differentiatetowards neural lineage in those lines derived from APOE4 carriers.Conclusions:Our preliminary data suggest intrinsic differences in the neural differentiation capacity between cell linesderived from APOE4 or APOE3 carrier subjects. Further experiments would contribute to elucidate novelpathogenic pathways associated with neurodegeneration and susceptible of therapeutic modulation, likelycontributing to the development of new effective drugs against AD.
YR 2023
FD 2023
LK https://hdl.handle.net/10630/27362
UL https://hdl.handle.net/10630/27362
LA eng
NO This study was supported by Instituto de Salud Carlos III (ISCiii) of Spain, co-financed by FEDER funds fromEuropean Union, through grants PI21/00915 (to AG) and PI21/00914 (to JV); by Junta de Andalucia throughConsejería de Economía y Conocimiento grants UMA20-FEDERJA-048 (to JAGL), PY18-RT-2233 (to AG) andUS-1262734 (to JV) co-financed by Programa Operativo FEDER 2014-2020, and Programa Operativo deEmpleo Juvenil SNGJ4-11 to LCP. Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 1 mar 2026