RT Journal Article
T1 Transcriptome Analysis and Intraspecific Variation in Spanish Fir (Abies pinsapo Boiss.)
A1 Ortigosa Peña, Francisco
A1 Ávila-Sáez, Concepción
A1 Rubio-Valverde, Lourdes
A1 Álvarez-Garrido, Lucía
A1 Carreira de la Fuente, José Antonio
A1 Cañas-Pendón, Rafael Antonio
A1 Cánovas-Ramos, Francisco Miguel
K1 Coníferas
AB Spanish fir (Abies pinsapo Boiss.) is an endemic, endangered tree that has been scarcely investigated at the molecular level. In this work, the transcriptome of Spanish fir was assembled, providing a large catalog of expressed genes (22,769), within which a high proportion were full-length transcripts (12,545). This resource is valuable for functional genomics studies and genome annotation in this relict conifer species. Two intraspecific variations of A. pinsapo can be found within its largest population at the Sierra de las Nieves National Park: one with standard green needles and another with bluish-green needles. To elucidate the causes of both phenotypes, we studied different physiological and molecular markers and transcriptome profiles in the needles. “Green” trees showed higher electron transport efficiency and enhanced levels of chlorophyll, protein, and total nitrogen in the needles. In contrast, needles from “bluish” trees exhibited higher contents of carotenoids and cellulose. These results agreed with the differential transcriptomic profiles, suggesting an imbalance in the nitrogen status of “bluish” trees. Additionally, gene expression analyses suggested that these differences could be associated with different epigenomic profiles. Taken together, the reported data provide new transcriptome resources and a better understanding of the natural variation in this tree species, which can help improve guidelines for its conservation and the implementation of adaptive management strategies under climatic change.
PB IOAP-MPDI
YR 2022
FD 2022-08-19
LK https://hdl.handle.net/10630/24821
UL https://hdl.handle.net/10630/24821
LA eng
NO Ortigosa F, Ávila C, Rubio L, Álvarez-Garrido L, Carreira JA, Cañas RA, Cánovas FM. Transcriptome Analysis and Intraspecific Variation in Spanish Fir (Abies pinsapo Boiss.). International Journal of Molecular Sciences. 2022; 23(16):9351. https://doi.org/10.3390/ijms23169351
NO This research was funded by research projects “Genómica funcional del pinsapo” and “Genómica del pinsapo: Estudios estructurales y funcionales” (grant numbers UMA18-FEDERJA-018 and P20-00507-R, funded by Junta de Andalucía, PAIDI) and “Modulators of adaptive capacity to climate change in forests: integration from the landscape to the gene/transcriptome in relict mountain conifers (CoMoReAdapt)” (grant number CGL2013-48843-C2-1-R, funded by the Spanish Ministry of Economy and Competitiveness, National Program for R + D+i). Partial funding for open access charge: Universidad de Málaga
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026